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Question #4 The graph on the left shows data for an experiment in which isolated mitochondria were suspended in buffered medium. In the experiment the ability of the mitochondria to consume oxygen (dashed lines) and produce ATP (solid lines) was monitored after the addition of various compounds. Complete the graph to show what happens (how the oxygen consumption and ATP production of the mitochondria change) at steps 1 to 4 in the experiment. In the boxes below briefly explain your answer for each step.

Question #4 The graph on the left shows data for an experiment in which isolated mitochondria were suspended in buffered medium. In the experiment the ability of the mitochondria to consume oxygen (dashed lines) and produce ATP (solid lines) was monitored after the addition of various compounds. Complete the graph to show what happens (how the oxygen consumption and ATP production of the mitochondria change) at steps 1 to 4 in the experiment. In the boxes below briefly explain your answer for each step.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Oxygen consumed Time 1 2 3 STEPS 4A Step 1. ADP+Pi is added 4B Step 2. Succinate is added 4C Step 3. ATP synthase inhibitor Oligomycin is added. 4D Step 4. The compound FCCP (Carbonyl-p- trifluoromethoxyphenylhrdrazone) is added. FCCP carries protons across the inner-membrane and dissipates any proton gradient. Finish Experiment 4 EXPLANATION ATP Synthesized Question #4 The graph on the left shows data for an experiment in which isolated mitochondria were suspended in buffered medium. In the experiment the ability of the mitochondria to consume oxygen (dashed lines) and produce ATP (solid lines) was monitored after the addition of various compounds. Complete the graph to show what happens (how the oxygen consumption and ATP production of the mitochondria change) at steps 1 to 4 in the experiment. In the boxes below briefly explain your answer for each step.
Transcribed Image Text:Oxygen consumed Time 1 2 3 STEPS 4A Step 1. ADP+Pi is added 4B Step 2. Succinate is added 4C Step 3. ATP synthase inhibitor Oligomycin is added. 4D Step 4. The compound FCCP (Carbonyl-p- trifluoromethoxyphenylhrdrazone) is added. FCCP carries protons across the inner-membrane and dissipates any proton gradient. Finish Experiment 4 EXPLANATION ATP Synthesized Question #4 The graph on the left shows data for an experiment in which isolated mitochondria were suspended in buffered medium. In the experiment the ability of the mitochondria to consume oxygen (dashed lines) and produce ATP (solid lines) was monitored after the addition of various compounds. Complete the graph to show what happens (how the oxygen consumption and ATP production of the mitochondria change) at steps 1 to 4 in the experiment. In the boxes below briefly explain your answer for each step.
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