Question 20 The following data were obtained for a hypothetical enzyme-catalysed reaction; S→P. (15 marks) [S] (M) 6.25 x 10-6 7.50 x 10-5 1.00 x 10-4 1.00 x 10-3 1.00 x 102 v (nmol/L/min) 15.0 56.25 60 74.9 75 = A. Is it reasonable to assume that Vmax 75 nmol/L/min? Justify your answer. (2) B. Calculate the KM value of the reaction shown above. (4) C. Calculate the values of ✓ when: i. [S] = 2.5 x 105 M and ii. [S] = 5x 105 M D. Comment on the nature of the relationship between the two values of v obtained above? E. By means of a calculation, determine v when [S] = 5.0 x105 M and the enzyme concentration is doubled. (4) (1) (3)

Question 20 The following data were obtained for a hypothetical enzyme-catalysed reaction; S→P. (15 marks) [S] (M) 6.25 x 10-6 7.50 x 10-5 1.00 x 10-4 1.00 x 10-3 1.00 x 102 v (nmol/L/min) 15.0 56.25 60 74.9 75 = A. Is it reasonable to assume that Vmax 75 nmol/L/min? Justify your answer. (2) B. Calculate the KM value of the reaction shown above. (4) C. Calculate the values of ✓ when: i. [S] = 2.5 x 105 M and ii. [S] = 5x 105 M D. Comment on the nature of the relationship between the two values of v obtained above? E. By means of a calculation, determine v when [S] = 5.0 x105 M and the enzyme concentration is doubled. (4) (1) (3)

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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