Question 16 Caffeine, benzoate, and aspartame content of mountain dew soda was determined using reverse phase HPLC. The concentration of the standard used are as follows, caffeine: 0.70 mg/mL, benzoate: 1.6 mg/mL, and aspartame 5.0 mg/mL. The following volume of the standards were taken to a 50.0 mL volumetric flask and diluted to the mark to construct a calibration curve Caffeine Benzoate Aspartame 1 mL 1 mL 1 mL 2 mL 2 mL 2 mL 3 mL 3 mL 3 mL 4 mL 4 mL 4 mL 5 mL 5 mL 5 mL The 12-oz can mountain dew sample were treated as follows: The 12- oz can were has been left out overnight to get rid the carbonation then, 2 mL were drawn into a plastic syringe, filtered, and placed into a vial. An equal amount of deionized water was added. A 100 μL sample were injected into a sample loop using same parameters with the standards. Assume 12 oz 354.9 mL The following data were obtained: Caffeine Peak Area Benzoate 3.54 Aspartame Std 1 2.30 3.33 ZZ Std 2 4.10 5.83 Std 3 7.37 9.63 Std 4 9.56 12.78 Std 5 12.18 16.33 Mountain Dew 10.21 7.96 Calculate the concentration of benzoate (mg/can) in the can sample. O A 190.866 mg/can OB. 142.186 mg/can OC. 47.716 mg/can OD. 95.433 mg/can 5.59 9.98 14.60 18.16 14.97

Question 16 Caffeine, benzoate, and aspartame content of mountain dew soda was determined using reverse phase HPLC. The concentration of the standard used are as follows, caffeine: 0.70 mg/mL, benzoate: 1.6 mg/mL, and aspartame 5.0 mg/mL. The following volume of the standards were taken to a 50.0 mL volumetric flask and diluted to the mark to construct a calibration curve Caffeine Benzoate Aspartame 1 mL 1 mL 1 mL 2 mL 2 mL 2 mL 3 mL 3 mL 3 mL 4 mL 4 mL 4 mL 5 mL 5 mL 5 mL The 12-oz can mountain dew sample were treated as follows: The 12- oz can were has been left out overnight to get rid the carbonation then, 2 mL were drawn into a plastic syringe, filtered, and placed into a vial. An equal amount of deionized water was added. A 100 μL sample were injected into a sample loop using same parameters with the standards. Assume 12 oz 354.9 mL The following data were obtained: Caffeine Peak Area Benzoate 3.54 Aspartame Std 1 2.30 3.33 ZZ Std 2 4.10 5.83 Std 3 7.37 9.63 Std 4 9.56 12.78 Std 5 12.18 16.33 Mountain Dew 10.21 7.96 Calculate the concentration of benzoate (mg/can) in the can sample. O A 190.866 mg/can OB. 142.186 mg/can OC. 47.716 mg/can OD. 95.433 mg/can 5.59 9.98 14.60 18.16 14.97

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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