Question 12 At high enough temperatures, hybridization is not spontaneous. This is because Ο ΤΔΗ>ΙASI O ITAHI = IASI %3D IAHI < ITASI ΙΔΗΙ > AS ΙΔΗ SI %3D O ITAHI < IASI Question 13 At the high temperature determined in Q12, if one starts with a heteroduplex, the heteroduplex will O come apart (melt) remain stable O partially come apart so that both double-stranded and single-stranded nucleic acids are present. Ouestion 14

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Question 12
At high enough temperatures, hybridization is not spontaneous. This is because
ΤΔΗ > ΙASI
ITAHI = IASI
%3D
IAHI < ITASI
ΙΔΗΙ > ΤASI
O IAHI = ITASI
%3D
O ITAHI < IASI
Question 13
At the high temperature determined in Q12, if one starts with a heteroduplex, the heteroduplex will
O come apart (melt)
O remain stable
O partially come apart so that both double-stranded and single-stranded nucleic acids are present.
Question 14
The reverse of hybridization is melting. The melting point is the temperature at which both double-stranded and single-
stranded nucleic acids are present. The melting point
O is the temperature at which IAHI = ITASI.
O is the temperature at which ITAHI = IASI.
can only be determined experimentally, as it is unrelated to free energy.
Transcribed Image Text:Question 12 At high enough temperatures, hybridization is not spontaneous. This is because ΤΔΗ > ΙASI ITAHI = IASI %3D IAHI < ITASI ΙΔΗΙ > ΤASI O IAHI = ITASI %3D O ITAHI < IASI Question 13 At the high temperature determined in Q12, if one starts with a heteroduplex, the heteroduplex will O come apart (melt) O remain stable O partially come apart so that both double-stranded and single-stranded nucleic acids are present. Question 14 The reverse of hybridization is melting. The melting point is the temperature at which both double-stranded and single- stranded nucleic acids are present. The melting point O is the temperature at which IAHI = ITASI. O is the temperature at which ITAHI = IASI. can only be determined experimentally, as it is unrelated to free energy.
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