Question #12 An actively respiring bacterial culture is supplied with pyruvate labeled on the methyl carbon with carbon-14 (carbon-14 is radioactive). Shortly after adding the label the citric acid intermediates are isolated and the position of the carbon-14 atom is determined. 12A Where is the carbon-14 label on alpha-ketoglutarate? Consider only the initial incorporation of carbon-14 in the first pass of labeled pyruvate through the citric acid cycle. [ draw the relevant structures that show how pyruvate is transformed to alpha-ketoglutarate. No mechanisms are required, but provide balanced equations. You do not need to draw the structures of cofactors] 12B. What enzyme(s) in the pathway that convert pyruvate to alpha-ketoglutarate are regulated?
Question #12 An actively respiring bacterial culture is supplied with pyruvate labeled on the methyl carbon with carbon-14 (carbon-14 is radioactive). Shortly after adding the label the citric acid intermediates are isolated and the position of the carbon-14 atom is determined. 12A Where is the carbon-14 label on alpha-ketoglutarate? Consider only the initial incorporation of carbon-14 in the first pass of labeled pyruvate through the citric acid cycle. [ draw the relevant structures that show how pyruvate is transformed to alpha-ketoglutarate. No mechanisms are required, but provide balanced equations. You do not need to draw the structures of cofactors] 12B. What enzyme(s) in the pathway that convert pyruvate to alpha-ketoglutarate are regulated?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Please help with parts a and b. If cant do all, then as much as you can answer. Thank you !
![### Question #12
An actively respiring bacterial culture is supplied with pyruvate labeled on the methyl carbon with carbon-14 (carbon-14 is radioactive). Shortly after adding the label the citric acid intermediates are isolated and the position of the carbon-14 atom is determined.
**12A:** Where is the carbon-14 label on alpha-ketoglutarate? Consider only the initial incorporation of carbon-14 in the first pass of labeled pyruvate through the citric acid cycle. [Draw the relevant structures that show how pyruvate is transformed to alpha-ketoglutarate. No mechanisms are required, but provide balanced equations. You do not need to draw the structures of cofactors.]
**12B:** What enzyme(s) in the pathway that convert pyruvate to alpha-ketoglutarate are regulated?](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F2d95edf5-a7eb-463b-b7f4-ca02808af034%2F71e2480a-5b12-41ee-98f3-bba9ec35d6ce%2Fo3zlyl_processed.jpeg&w=3840&q=75)
Transcribed Image Text:### Question #12
An actively respiring bacterial culture is supplied with pyruvate labeled on the methyl carbon with carbon-14 (carbon-14 is radioactive). Shortly after adding the label the citric acid intermediates are isolated and the position of the carbon-14 atom is determined.
**12A:** Where is the carbon-14 label on alpha-ketoglutarate? Consider only the initial incorporation of carbon-14 in the first pass of labeled pyruvate through the citric acid cycle. [Draw the relevant structures that show how pyruvate is transformed to alpha-ketoglutarate. No mechanisms are required, but provide balanced equations. You do not need to draw the structures of cofactors.]
**12B:** What enzyme(s) in the pathway that convert pyruvate to alpha-ketoglutarate are regulated?
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