Question 10. You work for the FDA and need to test a new drug for mutagenicity before it can go on the market. You set up an Ames Test with two different his strains of Salmonella as follows. Strain A has a single base pair substitution, while Strain B has two frameshift mutations. You include EMS and EtBr as positive controls. You plate cells from each strain onto minimal media plates lacking histidine and expose them to the drug. The table below shows the results of your Ames Test. Mutagen Strain A (# colonies per plate) Strain B (# colonies per plate) No mutagen (negative control) 40 10 EMS (positive control) 400 15 EtBr (positive control) 45 100 New drug 450 15 b) Based on these results, what do you report to the FDA about the mutagenicity of the new drug. Briefly explain your reasoning by stating which results support your interpretation. O Yes, the drug is mutagenic; there are many more colonies on Strain B with EtBr than there are on the negative control. O No, the drug is not mutagenic; there are many more colonies on Strain A than Strain B. O Yes, the drug is mutagenic; there are many more colonies on Strain B with the drug than there are on the negative control. O Yes, the drug is mutagenic; there are many more colonies on Strain A with the drug than the negative control. O No, the drug is not mutagenic; there are a similar number of colonies on Strain A with the new drug as the EMS control. O No, the drug is not mutagenic; there are a similar number of colonies on Strain B as the negative control.
Question 10. You work for the FDA and need to test a new drug for mutagenicity before it can go on the market. You set up an Ames Test with two different his strains of Salmonella as follows. Strain A has a single base pair substitution, while Strain B has two frameshift mutations. You include EMS and EtBr as positive controls. You plate cells from each strain onto minimal media plates lacking histidine and expose them to the drug. The table below shows the results of your Ames Test. Mutagen Strain A (# colonies per plate) Strain B (# colonies per plate) No mutagen (negative control) 40 10 EMS (positive control) 400 15 EtBr (positive control) 45 100 New drug 450 15 b) Based on these results, what do you report to the FDA about the mutagenicity of the new drug. Briefly explain your reasoning by stating which results support your interpretation. O Yes, the drug is mutagenic; there are many more colonies on Strain B with EtBr than there are on the negative control. O No, the drug is not mutagenic; there are many more colonies on Strain A than Strain B. O Yes, the drug is mutagenic; there are many more colonies on Strain B with the drug than there are on the negative control. O Yes, the drug is mutagenic; there are many more colonies on Strain A with the drug than the negative control. O No, the drug is not mutagenic; there are a similar number of colonies on Strain A with the new drug as the EMS control. O No, the drug is not mutagenic; there are a similar number of colonies on Strain B as the negative control.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Transcribed Image Text:Question 10. You work for the FDA and need to test a new drug for mutagenicity before it can go on
the market. You set up an Ames Test with two different his " strains of Salmonella as follows. Strain A
has a single base pair substitution, while Strain B has two frameshift mutations. You include EMS and
EtBr as positive controls. You plate cells from each strain onto minimal media plates lacking histidine
and expose them to the drug. The table below shows the results of your Ames Test.
Mutagen
Strain A (# colonies per plate)
Strain B (# colonies per plate)
No mutagen (negative control)
40
10
EMS (positive control)
400
15
EtBr (positive control)
45
100
New drug
450
15
b) Based on these results, what do you report to the FDA about the mutagenicity of the new drug.
Briefly explain your reasoning by stating which results support your interpretation.
O Yes, the drug is mutagenic; there are many more colonies on Strain B with EtBr than there are on the negative
control.
O No, the drug is not mutagenic; there are many more colonies on Strain A than Strain B.
O Yes, the drug is mutagenic; there are many more colonies on Strain B with the drug than there are on the negative
control.
O Yes, the drug is mutagenic; there are many more colonies on Strain A with the drug than the negative control.
O No, the drug is not mutagenic; there are a similar number of colonies on Strain A with the new drug as the EMS
control.
O No, the drug is not mutagenic; there are a similar number of colonies on Strain B as the negative control.
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