Q5. Ion exchange chromatography purifies a protein based on difference in magnitude of net Identify the type of column (anion exchange OR cation exchange) depicted in the illustration? add protein mixture wash off unbound proteins elute bound proteins with low salt buffer elute bound proteins with high salt buffer
Q5. Ion exchange chromatography purifies a protein based on difference in magnitude of net Identify the type of column (anion exchange OR cation exchange) depicted in the illustration? add protein mixture wash off unbound proteins elute bound proteins with low salt buffer elute bound proteins with high salt buffer
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:Q5.
Ion exchange chromatography purifies a protein based on difference in magnitude of net
Identify the type of column (anion exchange
OR cation exchange) depicted in the
illustration?
add protein mixture
wash off unbound proteins
elute bound proteins
with low salt buffer
elute bound proteins
with high salt buffer
Expert Solution

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Chromatography is the separation of a mixture of compounds into their individual components based on their relative interactions with an inert matrix.
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