Q5. Ion exchange chromatography purifies a protein based on difference in magnitude of net Identify the type of column (anion exchange OR cation exchange) depicted in the illustration? add protein mixture wash off unbound proteins elute bound proteins with low salt buffer elute bound proteins with high salt buffer

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Q5.
Ion exchange chromatography purifies a protein based on difference in magnitude of net
Identify the type of column (anion exchange
OR cation exchange) depicted in the
illustration?
add protein mixture
wash off unbound proteins
elute bound proteins
with low salt buffer
elute bound proteins
with high salt buffer
Transcribed Image Text:Q5. Ion exchange chromatography purifies a protein based on difference in magnitude of net Identify the type of column (anion exchange OR cation exchange) depicted in the illustration? add protein mixture wash off unbound proteins elute bound proteins with low salt buffer elute bound proteins with high salt buffer
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Chromatography is the separation of a mixture of compounds into their individual components based on their relative interactions with an inert matrix.

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