Provided in the Table below kinetic data for an enzymatic reaction that was carried out in the presence (last two columns) and absence (second column = control) of enzyme inhibitor. Both inhibitors were added in each reaction at a concentration of 2 mM. The enzyme concentration was similar in all and was approximately 0.001 иМ, a. Calculate both Vmax and KM for the control using Lineweaver-Burk curve. b. Provide the type of inhibition for both? (1) V° [(umol/(ml.s)][(umol/(ml.s)] | [(umol/(ml.s)] (2) V° (3) V° [S] (mM) 7.6 4.4 6.6 4 14.6 8.6 11.4 8. 26.6 16.4 17.8 16 45.8 29.8 24.6 24 60 40.8 28.2
Provided in the Table below kinetic data for an enzymatic reaction that was carried out in the presence (last two columns) and absence (second column = control) of enzyme inhibitor. Both inhibitors were added in each reaction at a concentration of 2 mM. The enzyme concentration was similar in all and was approximately 0.001 иМ, a. Calculate both Vmax and KM for the control using Lineweaver-Burk curve. b. Provide the type of inhibition for both? (1) V° [(umol/(ml.s)][(umol/(ml.s)] | [(umol/(ml.s)] (2) V° (3) V° [S] (mM) 7.6 4.4 6.6 4 14.6 8.6 11.4 8. 26.6 16.4 17.8 16 45.8 29.8 24.6 24 60 40.8 28.2
Chemistry
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ISBN:9781305957404
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Chapter1: Chemical Foundations
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Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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![Provided in the Table below kinetic data for an enzymatic reaction that was carried
out in the presence (last two
columns) and absence (second column = control) of enzyme inhibitor. Both
inhibitors were added in each
reaction at a concentration of 2 mM. The enzyme concentration was similar in all
and was approximately 0.001
иМ,
a. Calculate both Vmax and KM for the control using Lineweaver-Burk curve.
b. Provide the type of inhibition for both?
(1) V°
[(umol/(ml.s)][(umol/(ml.s)] | [(umol/(ml.s)]
(2) V°
(3) V°
[S] (mM)
7.6
4.4
6.6
4
14.6
8.6
11.4
8.
26.6
16.4
17.8
16
45.8
29.8
24.6
24
60
40.8
28.2](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F9f71af74-6195-4c4b-8605-98207032500d%2F43e83367-3c68-4264-aba6-41832bcf387c%2Fma2erek.png&w=3840&q=75)
Transcribed Image Text:Provided in the Table below kinetic data for an enzymatic reaction that was carried
out in the presence (last two
columns) and absence (second column = control) of enzyme inhibitor. Both
inhibitors were added in each
reaction at a concentration of 2 mM. The enzyme concentration was similar in all
and was approximately 0.001
иМ,
a. Calculate both Vmax and KM for the control using Lineweaver-Burk curve.
b. Provide the type of inhibition for both?
(1) V°
[(umol/(ml.s)][(umol/(ml.s)] | [(umol/(ml.s)]
(2) V°
(3) V°
[S] (mM)
7.6
4.4
6.6
4
14.6
8.6
11.4
8.
26.6
16.4
17.8
16
45.8
29.8
24.6
24
60
40.8
28.2
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