- Provide a point and interval estimate (95% CI) for the interlaboratory standard deviation of mean zone diameters for each type of control strain, assuming each laboratory uses different media to perform the  usceptibility tests. b- Answer Problem a assuming each laboratory uses a common medium to perform the susceptibility tests. c- Are there any advantages to using a common medium versus using different media for performing the susceptibility tests with regard to  tandardization of results across laboratories?

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a- Provide a point and interval estimate (95% CI) for the interlaboratory standard deviation of mean zone diameters for each type of control strain, assuming each laboratory uses different media to perform the  usceptibility tests.
b- Answer Problem a assuming each laboratory uses a common medium to perform the susceptibility tests.
c- Are there any advantages to using a common medium versus using different media for performing the susceptibility tests with regard to  tandardization of results across laboratories?

Microbiology
A nine-laboratory cooperative study was performed to
evaluate quality control for susceptibility tests with 30-µg
netilmicin disks [6]. Each laboratory tested three standard
control strains on a different lot of Mueller-Hinton agar, with
150 tests performed per laboratory. For protocol control,
each laboratory also performed 15 additional tests on each
of the control strains using the same lot of Mueller-Hinton
agar across laboratories. The mean zone diameters for each
of the nine laboratories are given in Table 6.11.
Transcribed Image Text:Microbiology A nine-laboratory cooperative study was performed to evaluate quality control for susceptibility tests with 30-µg netilmicin disks [6]. Each laboratory tested three standard control strains on a different lot of Mueller-Hinton agar, with 150 tests performed per laboratory. For protocol control, each laboratory also performed 15 additional tests on each of the control strains using the same lot of Mueller-Hinton agar across laboratories. The mean zone diameters for each of the nine laboratories are given in Table 6.11.
TABLE 6.11
Mean zone diameters with 30-µg netilmicin disks tested in nine separate laboratories
Type of control strain
E. coli
S. aureus
P. aeruginosa
Laboratory
Different media
Common medium
Different media
Common medium
Different media
Common medium
A
27.5
23.8
25.4
23.9
20.1
16.7
24.6
21.1
24.8
24.2
18.4
17.0
25.3
25.4
24.6
25.0
16.8
17.1
28.7
25.4
29.8
26.7
21.7
18.2
23.0
24.8
27.5
25.3
20.1
16.7
F
26.8
25.7
28.1
25.2
20.3
19.2
G
24.7
26.8
31.2
27.1
22.8
18.8
24.3
26.2
24.3
26.5
19.9
18.1
24.9
26.3
25.4
25.1
19.3
19.2
Transcribed Image Text:TABLE 6.11 Mean zone diameters with 30-µg netilmicin disks tested in nine separate laboratories Type of control strain E. coli S. aureus P. aeruginosa Laboratory Different media Common medium Different media Common medium Different media Common medium A 27.5 23.8 25.4 23.9 20.1 16.7 24.6 21.1 24.8 24.2 18.4 17.0 25.3 25.4 24.6 25.0 16.8 17.1 28.7 25.4 29.8 26.7 21.7 18.2 23.0 24.8 27.5 25.3 20.1 16.7 F 26.8 25.7 28.1 25.2 20.3 19.2 G 24.7 26.8 31.2 27.1 22.8 18.8 24.3 26.2 24.3 26.5 19.9 18.1 24.9 26.3 25.4 25.1 19.3 19.2
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