Prostaglandins are a class of eicosanoids, fatty acid derivatives involved in a variety of important phenomena, including fever, inflammation, and pain. The first step in prostaglandin synthesis involves the conversion of the 20-carbon fatty acid arachidonic acid to PGG2 by the enzyme prostaglandin endoperoxide synthase, or cyclooxy: Ibuprofen is one of several inhibitors of cyclooxogenase used therapeutically. Arachidonic acid 20₂ 0.5 1.0 1.5 2.5 3.5 cyclooxygenase PGG₂ JOH محمد Ibuprofen The following kinetic data were obtained for the enzyme cyclooxygenase in the absence of any inhibitor (1), and in the presence of ibuprofen (2) at a concentration of 48 [arachidonic acid] (uM) (1) v (uM/min) (2) v (uM/min) 23.5 16.67 32.2 25.25 36.9 30.49 41.8 37.04 44.0 38.91 A. Plot the data in standard Michaelis-Menten form as well as in double-reciprocal form. B. Determine Vmax and Km for the enzyme. C. Based on this limited data set, what type of inhibition does ibuprofen likely exhibit? Calculate the relevant inhibition constant(s) for ibuprofen.
Prostaglandins are a class of eicosanoids, fatty acid derivatives involved in a variety of important phenomena, including fever, inflammation, and pain. The first step in prostaglandin synthesis involves the conversion of the 20-carbon fatty acid arachidonic acid to PGG2 by the enzyme prostaglandin endoperoxide synthase, or cyclooxy: Ibuprofen is one of several inhibitors of cyclooxogenase used therapeutically. Arachidonic acid 20₂ 0.5 1.0 1.5 2.5 3.5 cyclooxygenase PGG₂ JOH محمد Ibuprofen The following kinetic data were obtained for the enzyme cyclooxygenase in the absence of any inhibitor (1), and in the presence of ibuprofen (2) at a concentration of 48 [arachidonic acid] (uM) (1) v (uM/min) (2) v (uM/min) 23.5 16.67 32.2 25.25 36.9 30.49 41.8 37.04 44.0 38.91 A. Plot the data in standard Michaelis-Menten form as well as in double-reciprocal form. B. Determine Vmax and Km for the enzyme. C. Based on this limited data set, what type of inhibition does ibuprofen likely exhibit? Calculate the relevant inhibition constant(s) for ibuprofen.
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
Related questions
Concept explainers
Analyzing Infrared Spectra
The electromagnetic radiation or frequency is classified into radio-waves, micro-waves, infrared, visible, ultraviolet, X-rays and gamma rays. The infrared spectra emission refers to the portion between the visible and the microwave areas of electromagnetic spectrum. This spectral area is usually divided into three parts, near infrared (14,290 – 4000 cm-1), mid infrared (4000 – 400 cm-1), and far infrared (700 – 200 cm-1), respectively. The number set is the number of the wave (cm-1).
IR Spectrum Of Cyclohexanone
It is the analysis of the structure of cyclohexaone using IR data interpretation.
IR Spectrum Of Anisole
Interpretation of anisole using IR spectrum obtained from IR analysis.
IR Spectroscopy
Infrared (IR) or vibrational spectroscopy is a method used for analyzing the particle's vibratory transformations. This is one of the very popular spectroscopic approaches employed by inorganic as well as organic laboratories because it is helpful in evaluating and distinguishing the frameworks of the molecules. The infra-red spectroscopy process or procedure is carried out using a tool called an infrared spectrometer to obtain an infrared spectral (or spectrophotometer).
Question
![Prostaglandins are a class of eicosanoids, fatty acid derivatives involved in a variety of important phenomena, including fever, inflammation, and pain. The first step in
prostaglandin synthesis involves the conversion of the 20-carbon fatty acid arachidonic acid to PGG2 by the enzyme prostaglandin endoperoxide synthase, or cyclooxy:
Ibuprofen is one of several inhibitors of cyclooxogenase used therapeutically.
Arachidonic acid
20₂
0.5
1.0
1.5
2.5
3.5
cyclooxrygenase
PGG₂
JOH
محمد
The following kinetic data were obtained for the enzyme cyclooxygenase in the absence of any inhibitor (1), and in the presence of ibuprofen (2) at a concentration of 48
[arachidonic acid] (uM)
(1) v(uM/min) (2) v(uM/min)
23.5
32.2
36.9
41.8
44.0
16.67
25.25
30.49
37.04
38.91
Ibuprofen
A. Plot the data in standard Michaelis-Menten form as well as in double-reciprocal form.
B. Determine Vmax and Km for the enzyme.
C. Based on this limited data set, what type of inhibition does ibuprofen likely exhibit? Calculate the relevant inhibition constant(s) for ibuprofen.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F7029a629-741a-46d0-bb00-9a5c18adacbb%2Fbb6795bf-f2bf-40d4-98e3-bf3b9b0a1908%2Ffzu049n_processed.jpeg&w=3840&q=75)
Transcribed Image Text:Prostaglandins are a class of eicosanoids, fatty acid derivatives involved in a variety of important phenomena, including fever, inflammation, and pain. The first step in
prostaglandin synthesis involves the conversion of the 20-carbon fatty acid arachidonic acid to PGG2 by the enzyme prostaglandin endoperoxide synthase, or cyclooxy:
Ibuprofen is one of several inhibitors of cyclooxogenase used therapeutically.
Arachidonic acid
20₂
0.5
1.0
1.5
2.5
3.5
cyclooxrygenase
PGG₂
JOH
محمد
The following kinetic data were obtained for the enzyme cyclooxygenase in the absence of any inhibitor (1), and in the presence of ibuprofen (2) at a concentration of 48
[arachidonic acid] (uM)
(1) v(uM/min) (2) v(uM/min)
23.5
32.2
36.9
41.8
44.0
16.67
25.25
30.49
37.04
38.91
Ibuprofen
A. Plot the data in standard Michaelis-Menten form as well as in double-reciprocal form.
B. Determine Vmax and Km for the enzyme.
C. Based on this limited data set, what type of inhibition does ibuprofen likely exhibit? Calculate the relevant inhibition constant(s) for ibuprofen.
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
Step by step
Solved in 5 steps with 5 images
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.Recommended textbooks for you
Chemistry
Chemistry
ISBN:
9781305957404
Author:
Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:
Cengage Learning
Chemistry
Chemistry
ISBN:
9781259911156
Author:
Raymond Chang Dr., Jason Overby Professor
Publisher:
McGraw-Hill Education
Principles of Instrumental Analysis
Chemistry
ISBN:
9781305577213
Author:
Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:
Cengage Learning
Chemistry
Chemistry
ISBN:
9781305957404
Author:
Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:
Cengage Learning
Chemistry
Chemistry
ISBN:
9781259911156
Author:
Raymond Chang Dr., Jason Overby Professor
Publisher:
McGraw-Hill Education
Principles of Instrumental Analysis
Chemistry
ISBN:
9781305577213
Author:
Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:
Cengage Learning
Organic Chemistry
Chemistry
ISBN:
9780078021558
Author:
Janice Gorzynski Smith Dr.
Publisher:
McGraw-Hill Education
Chemistry: Principles and Reactions
Chemistry
ISBN:
9781305079373
Author:
William L. Masterton, Cecile N. Hurley
Publisher:
Cengage Learning
Elementary Principles of Chemical Processes, Bind…
Chemistry
ISBN:
9781118431221
Author:
Richard M. Felder, Ronald W. Rousseau, Lisa G. Bullard
Publisher:
WILEY