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Procedure A Take 3 ml Top up to S0ml, Add 50 of Mix Sml mixture analyse to - Add fresh LB ampicillin to the mixture overnight culture- with 45 ml LB the both broth OD600 The mixture is then Withdraw 3ml mixture to Measure every 15 mins. Stop until 75 mins. Overnight culture analyse (OD600)record as zero time-point. - incubated at shaked (E-coli LB borth) incubator at 37°c 200rpm Mix overnight culture B (control) with 45 ml LB Sml Add 50 ul of ampicillin to the Take 3 ml mixture to No ampicilin analyse O0600 the mixture broth The mixture is incubated incubator at 37c 200rpm Withdraw 3ml mixture to analyse (OD600)record as zero time-point. then Measure every 15 mins. Stop until 75 mins at shaked

Procedure A Take 3 ml Top up to S0ml, Add 50 of Mix Sml mixture analyse to - Add fresh LB ampicillin to the mixture overnight culture- with 45 ml LB the both broth OD600 The mixture is then Withdraw 3ml mixture to Measure every 15 mins. Stop until 75 mins. Overnight culture analyse (OD600)record as zero time-point. - incubated at shaked (E-coli LB borth) incubator at 37°c 200rpm Mix overnight culture B (control) with 45 ml LB Sml Add 50 ul of ampicillin to the Take 3 ml mixture to No ampicilin analyse O0600 the mixture broth The mixture is incubated incubator at 37c 200rpm Withdraw 3ml mixture to analyse (OD600)record as zero time-point. then Measure every 15 mins. Stop until 75 mins at shaked

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Write a short discussion based on the graph and calculate mean growth rate and mean generation time

Procedure A Add 50 ul of Take 3 ml mixture Mix Sml Top up to S0ml, to - Add fresh LB ampicillin to the overnight culture- mixture with 45 ml La analyse the both broth OD600 Withdraw 3ml mixture to The mixture is then at shaked Measure every 15 mins. Stop until incubated 75 mins. Overnight culture (E-coli LB borth) analyse (OD600)record as zero time-point. incubator at 37°c 200rpm Mix overnight culture B (control) with 45 ml LB Sml Take 3 ml Add 50 ul of ampicillin to the mixture analyse O0600 to No ampicilin the mixture broth Measure every 15 mins. Stop until 75 mins. The mixture is then incubated at Withdraw 3ml mixture to analyse (OD600)record as zero time-point. shaked- incubator at 37c 200rpm Graph of Absorbance vs Time(mins) 1.4 1.2 0.8 0.6 0.4 0.2 10 20 30 40 50 60 70 80 Time (mins) Group 1 OD 600 (A) (B) Group 1 (Ampicilin) Group 1 (Control) Ampicillin 0.124 Control Time (minutes 0.248 15 0.756 0367 30 0.645 0.428 45 0.633 0.695 60 0.599 0.937 75 0.593 1.301 Absorbance(600nm)
Transcribed Image Text:Procedure A Add 50 ul of Take 3 ml mixture Mix Sml Top up to S0ml, to - Add fresh LB ampicillin to the overnight culture- mixture with 45 ml La analyse the both broth OD600 Withdraw 3ml mixture to The mixture is then at shaked Measure every 15 mins. Stop until incubated 75 mins. Overnight culture (E-coli LB borth) analyse (OD600)record as zero time-point. incubator at 37°c 200rpm Mix overnight culture B (control) with 45 ml LB Sml Take 3 ml Add 50 ul of ampicillin to the mixture analyse O0600 to No ampicilin the mixture broth Measure every 15 mins. Stop until 75 mins. The mixture is then incubated at Withdraw 3ml mixture to analyse (OD600)record as zero time-point. shaked- incubator at 37c 200rpm Graph of Absorbance vs Time(mins) 1.4 1.2 0.8 0.6 0.4 0.2 10 20 30 40 50 60 70 80 Time (mins) Group 1 OD 600 (A) (B) Group 1 (Ampicilin) Group 1 (Control) Ampicillin 0.124 Control Time (minutes 0.248 15 0.756 0367 30 0.645 0.428 45 0.633 0.695 60 0.599 0.937 75 0.593 1.301 Absorbance(600nm)
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