Problem 1. Mitochondria may be prepared from cells and tested in cell free experiments. If supplied with pyruvate and oxygen, isolated mitochondria are capable of aerobic metabolism. A. Describe how the rate of oxygen consumption is the rate of aerobic metabolism. B. You as the investigator measure the rate of oxygen consumption. You add the following inhibitors and compare the rate to a control that has no additions. Explain how each of the following results may have occurred based on a comparison of all results (Hint: focus on the relative numbers given below and not on the exact values above 0%). 1. You add an inhibitor of Complex I. The rate of oxygen consumption is decreased by 50%. 2. You add an inhibitor of Complex II. The rate of oxygen consumption is decreased 25%. 3. You add an inhibitor of Complex III. The rate of oxygen consumption is decreased 100%. 4. An uncoupler of oxidative phosphorylation allows the protons to return to the matrix of the mitochondria without going through the ATP synthase complex. (The brown fat mitochondria of bears use this mechanism to generate heat during hibernation. You add an uncoupler of oxidative phosphorylation.) Predict whether to expect the rate of oxygen consumption to increase or decrease from 100%. Explain.
Problem 1. Mitochondria may be prepared from cells and tested in cell free experiments. If supplied with pyruvate and oxygen, isolated mitochondria are capable of aerobic metabolism. A. Describe how the rate of oxygen consumption is the rate of aerobic metabolism. B. You as the investigator measure the rate of oxygen consumption. You add the following inhibitors and compare the rate to a control that has no additions. Explain how each of the following results may have occurred based on a comparison of all results (Hint: focus on the relative numbers given below and not on the exact values above 0%). 1. You add an inhibitor of Complex I. The rate of oxygen consumption is decreased by 50%. 2. You add an inhibitor of Complex II. The rate of oxygen consumption is decreased 25%. 3. You add an inhibitor of Complex III. The rate of oxygen consumption is decreased 100%. 4. An uncoupler of oxidative phosphorylation allows the protons to return to the matrix of the mitochondria without going through the ATP synthase complex. (The brown fat mitochondria of bears use this mechanism to generate heat during hibernation. You add an uncoupler of oxidative phosphorylation.) Predict whether to expect the rate of oxygen consumption to increase or decrease from 100%. Explain.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:Problem 1. Mitochondria may be prepared from cells and tested
in cell free experiments. If supplied with pyruvate and oxygen,
isolated mitochondria are capable of aerobic metabolism.
A. Describe how the rate of oxygen consumption is the rate of
aerobic metabolism.
B. You as the investigator measure the rate of oxygen
consumption. You add the following inhibitors and
compare the rate to a control that has no additions. Explain
how each of the following results may have occurred based
on a comparison of all results (Hint: focus on the relative
numbers given below and not on the exact values above
0%).
1. You add an inhibitor of Complex I. The rate of oxygen
consumption is decreased by 50%.
2. You add an inhibitor of Complex II. The rate of oxygen
consumption is decreased 25%.
3. You add an inhibitor of Complex III. The rate of oxygen
consumption is decreased 100%.
4. An uncoupler of oxidative phosphorylation allows the
protons to return to the matrix of the mitochondria
without going through the ATP synthase complex. (The
brown fat mitochondria of bears use this mechanism to
generate heat during hibernation. You add an uncoupler
of oxidative phosphorylation.) Predict whether to expect
the rate of oxygen consumption to increase or decrease
from 100%. Explain.
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