Prepare 1.0 L of a 0.1 M histidine buffer, pH 7.0, starting with histidine dihydrochloride (C3H11N3O2Cl2, FW 228), 0.5 N NaOH, and water. pKa values for histidine are 1.8 (α-COOH), 9.3 (α-NH3+), and 6.0 (-R).

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Prepare 1.0 L of a 0.1 M histidine buffer, pH 7.0, starting with histidine dihydrochloride (C3H11N3O2Cl2, FW 228), 0.5 N NaOH, and water. pKa values for histidine are 1.8 (α-COOH), 9.3 (α-NH3+), and 6.0 (-R).
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