Prelab Questions: 1. For each of the following four scenarios, assess if the random and systematic errors are large or small. Briefly explain your reasoning for each case based on the evidence provided by the target's image. A B C D
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- River water analysis of mercury (Hg) yields a spectrophotometric sample signal of 0.712 for a 5.00 mL sample. A 5.OuL spike of 1560 ppb Hg standard produced a signal of 1546. What is the concentration of Hg in the original river water sample? What is the concentration of Hg in the original river water sample? A. 1.109 ppb B. 1.330 ppb C. 1.771 ppb D. 1.546 ppba. Briefly explain why internal standardization method is useful inanalytical chemistry?b. Why does a response factor of an instrument’s detector need to becalculated?If the TLC plates had been developed in a more polar solvent, would retention factors on the whole be larger, smaller, or unchanged? Briefly (1 - 2 sentences) explain your reasoning
- If the peak width w of both Peak 1 and Peak 2 fro eon 1g is 0.5 minutes, what is the resolution factor between the two peaks? 1.13 Selectivity is Peak 1 (6.0 min, Peak 2 w=0.5 (6.8 min, w=0.5 25 min) min) 20 15 Hold up peak (1.8 min) 10 0. 0. 4. 8. time (minutes) 2. 5. SignalHow would a 95% Z-interval compare to a 95% T-interval?Table 1: Chromatography Results Mobile Phase: Distilled Water Solvent front distance (cm): 8 cm M&M Candy Separated Spot Color Migration Distance (cm) (distance to center of spot from origin) Red M&M Red 7.5 cm Green M&M Yellow 5.3 cm Blue 7.7 cm Blue M&M Blue 7.8 cm -please help with this part Calculations/Results: Table 1: Results with Distilled Water M&M Candy Separated Spot Color Rf Value Red M&M Green M&M Blue M&M Rf = Distance traveled by component in a given time/ Distance traveled by solvent in same time Show work with units for one sample calculation.
- Draw an HPLC instrument diagram by writing the name of the parts. Define the isocratic and gradient system. Write correctly the elution order of the compounds which are shown in the HPLC chromatogram below. Chromatogram Compound B These are called chromatographic peaks and each one represents a separated compound Compound C Point of sample injection into the column Compound AHow would the resolution (separation into discrete peaks) be affected if you used a column that was half as long? Resolution would be better Resolution would be worse Resolution would not changeDetermine the slope of the linear regression line for a standard curve of concentration versus absorbance using the following data. Concentration (M) Absorbance 2.00x10-5 0.0500 5.00x10-5 0.130 1.00x10-4 0.200 2.00x10-4 0.450 5.00x10-4 1.25 7.50x10-4 1.40 Group of answer choice 5.00x10-4 1.00x10-5 1990 0.0420 0.456 0.296
- Given the following spectrophotometric data, what is the most appropriate linear equation to use? Concentration, M. Abs 0.000 (Blank) 0.021 0.100 0.193 0.250 0.322 0.350 0465 0.500 0.668 0.750 0.801 1.00 0.955NAME: LAB SECTION: EXPERIMENT 21: Spectroscopy of Dyes POSTLAB EXERCISE 1. AUV/V is a spectrometer used to measure the absorbance of a solution of hemoglobin bound to oxy- gen at 590 nm. The molar absorptivity was determined to be 8.57 × 104 M-'cm-. The cuvette used had a 1.00-cm sample pathlength. The measured absorbance was ), 140. What was the concentra- tion of hemoglobin in the solution? 2. What would be the absorbance of the solution in question 1 if the pathlength were 2.00 cm? nohntooote 196Tap water is measured for arsenic and found to have a mean signal of 0.0061. Next, a small amount of Arsenic is spiked into the tap water such that the new value is 0.700 ppb higher than before. The mean signal from this new low-level concentration is 0.0447, with a standard deviation of 0.00560. What is the Lower Limit of Quantitation? (Hint: estimate slope as fraction numerator triangle Y over denominator triangle X end fraction , where triangle Y is the difference between the low level spike and the blank signals; and triangle X is the concentration change between the spiked and unspiked sample)