Please draw a concept map using the following terms for regulation  Repressor, Inducer, Induction, Negative regulation, Repression, Operator, Positive Regulation, Activator, Sigma-32, Sensor Kinase, Two-componenet Regulation, Lac operon, Heat shock, Catabolite repression, trp operon, Feedback inhibition, Protein Stability, Sigma factors, Response regulator, Riboswitch, Homoserine Lactone, Quorum sensing, Autoinducing Peptide, RNA regulation, Antisense RNA

Binding of _______ to _______ in DNA can increase the rate of transcription of specific genes. a. activators; repressors c. repressors; operators b. activators; enhancers d. repressors; enhancers

Which of the following is an example of positive control? (a) transcription occurs when a repressor binds to an inducer (b) transcription cannot occur when a repressor binds to a corepressor (c) transcription is stimulated when an activator protein binds to DNA (d) a and b (e) a and c

Binding or ___ to ___ in DNA can increase the rate of transcription of specific genes. a. activators; repressors b. activators; enhancers c. repressors: operators d. repressors: enhancers

Control of gene expression in eukaryotic cells occurs at which level(s)? a. only the transcriptional level b. epigenetic and transcriptional levels c. epigenetic, transcriptional, and translational levels d. epigenetic, transcriptional, post-transcriptional, translational, and post-translational levels

In selecting target cells to receive a transferred gene in gene therapy, what factors do you think would have to be taken into account?

PREDICT Compare the types of bacterial genes associated with inducible operons, those associated with repressible operons, and those that are constitutive. Predict the category into which each of the following would most likely fit: (a) a gene that codes for RNA polymerase, (b) a gene that codes for an enzyme required to break down maltose, and (c) a gene that codes for an enzyme used in the synthesis of adenine.

Through alternative splicing, eukaryotes (a) reinforce gene inactivation (b) prevent transcription of heterochromatin (c) produce related but different proteins in different tissues (d) amplify genes to meet the requirement of high levels of a gene product (e) bind transcription factors to enhancers to activate transcription

For the E. coli lac operon, when lactose is present: a. and glucose is absent, cAMP binds and activates catabolicactivator protein (CAP). b. and glucose is absent, the level of cAMP decreases. c. activated CAP binds the repressor protein to remove it from the operator gene. d. the cell prefers lactose over glucose. e. RNA polymerase cannot bind to the promoter.

Antibiotics and Protein Synthesis Antibiotics are molecules produced by microorganisms as defense mechanisms. The most effective antibiotics work by interfering with essential biochemical or reproductive processes. Many antibiotics block or disrupt one or more stages in protein synthesis. Some of these are mentioned here. Tetracyclines are a family of chemically related compounds used to treat several types of bacterial infections. Tetracyclines interfere with the initiation of translation. The tetracycline molecule attaches to the small ribosomal subunit and prevents binding of the tRNA anticodon during initiation. Both eukaryotic and prokaryotic ribosomes are sensitive to the action of tetracycline, but this antibiotic cannot pass through the plasma membrane of eukaryotic cells. Because tetracycline can enter bacterial cells to inhibit protein synthesis, it will stop bacterial growth, helping the immune system fight the infection. Streptomycin is used in hospitals to treat serious bacterial infections. It binds to the small ribosomal subunit but does not prevent initiation or elongation; however, it does affect the efficiency of protein synthesis. Binding of streptomycin changes the way mRNA codons interact with the tRNA. As a result, incorrect amino acids are incorporated into the growing polypeptide chain, producing nonfunctional proteins. In addition, streptomycin causes the ribosome to randomly fall off the mRNA, preventing the synthesis of complete proteins. Puromycin is not used clinically but has played an important role in studying the mechanism of protein synthesis in the research laboratory. The puromycin molecule is the same size and shape as a tRNA/amino acid complex. When puromycin enters the ribosome, it can be incorporated into a growing polypeptide chain, stopping further synthesis because no peptide bond can be formed between puromycin and an amino acid, causing the shortened polypeptide to fall off the ribosome. Chloramphenicol was one of the first broadspectrum antibiotics introduced. Eukaryotic cells are resistant to its actions, and it was widely used to treat bacterial infections. However, its use is limited to external applications and serious infections. Chloramphenicol destroys cells in the bone marrow, the source of all blood cells. In bacteria, this antibiotic binds to the large ribosomal subunit and inhibits the formation of peptide bonds. Another antibiotic, erythromycin, also binds to the large ribosomal subunit and inhibits the movement of ribosomes along the mRNA. Almost every step of protein synthesis can be inhibited by one antibiotic or another. Work on designing new synthetic antibiotics to fight infections is based on our knowledge of how the nucleotide sequence of mRNA is converted into the amino acid sequence of a protein. Questions Why is targeting protein synthesis an effective strategy for preventing infection?