Phosphate buffered saline (PBS) is a very common reagent in cell biology labs that do cell culture since is it osmotically stable for most eukaryotic cells. It is often prepared as 10X PBS (10.5 mM KH2PO4, 1.552 M NaCl, 29.7 mM Na2HPO4) to save on shelf space. How would you prepare 1 L of 10X PBS given 1M KH2PO4, NaCl (FW 58.44), 906mM Na2HPO4? a. What do you know about the forms of thechemicals you are given to prepare the 10X PBS and how do you know this? What should the final units for each of the 3 components be in your answer? What is the solvent for 10X PBS and how do you know this?

Q: The freezing point of water H,0 is 0.00°C at 1 atmosphere. A nonvolatile, nonelectrolyte that…

A: Introduction: The lowering of freezing temperature when a non-volatile solute is added to the…

Q: What mass of salt (NaCl) should you add to 1.62 L of water in an ice cream maker to make a solution…

A: Given: The volume and density of water is 1.62 L and 1.00 g/mL, respectively. The freezing point of…

Q: The molecular weight of glucose is 180g/mol. To prepare a stock solution of glucose, you dissolve…

A: First of all we have to calculate the concentration of the solution. From that we can calculate the…

Q: How much NaCl would you dissolve and dilute to the 100.00 mL mark in a volumetric flask with DI H2O…

A: Given,Molarity of NaCl = 0.475MThe amount of NaCl required to dilute to 100mL using water to prepare…

Q: Calculate the molarity of a solution prepared by dissolving 5.881 g NaCl in 250.0 mL of water. (MM…

A: Given : mass of NaCl = 5.881 g And volume of water = 250 mL.

Q: 5. If 152 g of Na2SO4 is dissolved in 875 g of H₂O, what is the boiling point of the solution?…

A: In the given question->Weight of Na2SO4 = 152 gmWeight of H2O = 875 gm = 0.875 kg       (1kg =…

Q: If the solubility of potassium chloride at 25 Celsius is 34.0 grams/ 100 ml of water, how many g of…

A: Use the dimensional analysis to calculate the mass of KCl in 322 ml of water and round it off to…

Q: 23. What is the concentration (by mass) of the solute in a 25 °C solution prepared by dissolving…

A: According to question  No. Of moles = 2.50× 10-7  Volume = 1L  So, Molarity = no. Of moles/Volume(L)…

Q: What is the molality of a solution that consists of 8.09 mL of carbon tetrachloride (CCl4, d = 1.59…

A:

Q: At 20 °C, sugar has a solubility of 203.9 g, how much water we need at minimum to dissolve two (2)…

A: Given, At 20 °C, sugar has a solubility of 203.9 g, The minimum amount of water need  to dissolve…

Q: What is the molarity of a solution prepared by dissolving 2.026 g of FeCl3 in 0.150 L of water ?

A:

Q: An effective germicide used in laboratories is a 70.0% v/v ethanol solution. This (v/v) means the…

A: We have to use unitary method for this.

Q: Ethylene glycol is the primary component in antifreeze. How many grams of ethylene glycol…

A: Molarity ( M ) is defined as " the number of moles of solute ( n ) present in one litre of solution.…

Q: (Q65) Calculate the vapor pressure (in torr) at 30°C of a solution containing 120.4 grams of the…

A:

Q: Sodium glutamate (commonly known as monosodium glutamate, MSG, with a molar mass of 169.11 g/mol) is…

A: Molar mass of MSG = 169.11 g/molMolarity of the MSG = 0.0858 M1 M = 1 mol/LVolume of solution = 1.92…

Q: A solution is prepared with 70.0 g of HNO3 and 130.0 g of H₂O. It has a density of 1.21 g/ml. What…

A: As you have posted multiple questions so we are answering first question for you for remaining…

Q: How many grams of Co(CH3COO)₂ are there in 197 grams of an aqueous solution that is 9.60 % by weight…

A: >mass/weight percent is defined as the mass of solute dissolved in 100 g of solution The formula…

Q: When 5.0 g of sodium phosphate dissolves in 50.0 mL of water, what is the boiling point of the…

A:

Q: Hexane (C6H14) is used as an organic solvent according to US EPA, there is no information its…

A:

Q: How much NaCl would you dissolve and dilute to the 100.00 mL mark in a volumetric flask with DI H2O…

A: Molar mass of NaCl = 58.44 g/mol

Q: An aqueous solution of ethanol (CH3CH2OH, 46.07 g mol–1 and H2O, 18.02 g mol–1) is 25.0% ethanol by…

A: In this problem, we are given the following information:The solution is 25.0% ethanol by mass. This…

Q: w many grams of glycerine, C3H5(OH) (MM = 92 g/mol) is mixed with 36 g H₂O to make a solution where…

A: Mole can be defined as a standard unit for measuring large quantities of very small entities such as…

Q: 142.04 g/mol) is needed to prepare 350. mL of a solution What mass of Na2SO4 (molar mass = having a…

A:

Q: What is the concentration in molarity of a solution which is 11.39 %m/v octane (MM = 114.2 g/mol )…

A: Molarity is defined as total number of moles of solute present in 1000 ml of solution Molarity =…

Q: What is the molality of a solution prepared by dissolving 18.5 g of sodium nitrate (MW=84.99g/mol)…

A:

Q: Nicotinamide is a water-soluble nonelectrolyte vitamin important in metabolism. Addition of 3.88 g…

A:

Q: How many milimeters of a 0.29 M sucrose solution contain 335g of sucrose (C12H22O11) ?

A:

Q: How many grams of Na3PO4 is actually produced? -Calculate the mass of NaH2PO4 needed to produce this…

A: Given data,Percent yield=82.5%Molarity of Na3PO4=0.55MVolume of Na3PO4=186.50mL=0.1865LMolarity of…

Q: A soft drink contains 50mg of sodium in 301g of H2O. What is the concentration of sodium in the soft…

A: Mass percent is given by, Mass percentage = Mass of the soluteMass of the solution×100

Q: What are the mole fraction and the mass percent of a solu-tion made by dissolving 0.30 g of KI in…

A: Substitute 0.4 L as volume and I g/mL as the density of water in the expression of density to…

Q: What is the mass percent of a solution made by adding 75.3 grams of KCl to 500 mL of water? (The…

A: Given: Mass of KCl = 75.3 g Volume of water =  500 mL Density of water = 1.00 g/mL

Q: 1. By referring to figure below, determine whether the addition of 40.0 g of each of the following…

A: The amount of solute present in 100 g of solvent is known as solubility. The solubility of a solute…

Q: How much NaCl would you dissolve and dilute to the 100.00 mL mark in a volumetric flask with DI H2O…

A: This question can be solves using molarity equation by directly putting the given value.

Solutions

Solutions can be considered as homogeneous mixtures which contain two or more than two chemical substances. The chemical substance which is the greater part is defined as solvent. And the chemical substance that is dissolved in a solvent is referred to as solute. Solution chemistry plays an important role in chemistry since it supports many commercial applications of solutions.