Penicillin is hydrolyzed and thereby rendered inactive by beta-lactamase, an enzyme present in some penicillin-resistant bacteria. The mass of this enzyme is 30.0 kDa. The amount of penicillin hydrolyzed in 1 minute in a 1 mL solution containing 3 x 10-3 g of the enzyme was measured as a function of the concentration of penicillin. Calculate Km, Vmax , and kcat from the following data: (Clearly show where 1/Vmax and -1/Km are; clearly explain how to obtain your kcat value). [S] (mM) vo (mM s-1) 0.14 0.138 0.28 0.234 0.55 0.333 0.77 0.388 1.52 0.491
Penicillin is hydrolyzed and thereby rendered inactive by beta-lactamase, an enzyme present in some penicillin-resistant bacteria. The mass of this enzyme is 30.0 kDa. The amount of penicillin hydrolyzed in 1 minute in a 1 mL solution containing 3 x 10-3 g of the enzyme was measured as a function of the concentration of penicillin. Calculate Km, Vmax , and kcat from the following data: (Clearly show where 1/Vmax and -1/Km are; clearly explain how to obtain your kcat value).
[S] (mM) vo (mM s-1)
0.14 0.138
0.28 0.234
0.55 0.333
0.77 0.388
1.52 0.491
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