Part A. Weight of skimmed milk Isolation of Casein Weight of dried casein Percentage casein in skimmed milk Part B. Identification Tests of Casein 1. Qualitative Tests 1.1 Biuret test. Substance 2% glycine 2% gelatin 2% albumin 1% tyrosine casein + distilled water Observed Color 8 09 6.0 8 %
Part A. Weight of skimmed milk Isolation of Casein Weight of dried casein Percentage casein in skimmed milk Part B. Identification Tests of Casein 1. Qualitative Tests 1.1 Biuret test. Substance 2% glycine 2% gelatin 2% albumin 1% tyrosine casein + distilled water Observed Color 8 09 6.0 8 %
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
Direction: Answer these questions thoroughly please and explain. Thank you so much.
Topic: Isolation and Characterization of Casein
![Part A.
Weight of skimmed milk
Isolation of Casein
Weight of dried casein
Percentage casein in skimmed milk
Part B.
1.
Identification Tests of Casein
Qualitative Tests
1.1 Biuret test.
Substance
2% glycine
2% gelatin
2% albumin
1% tyrosine
casein + distilled water
Observed Color
6.0
6.0
%](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F531ce7ca-2de8-48a7-aa83-6b0d470c3414%2F0d2dd9bd-ec00-4016-a428-2ecf5e450b41%2Fzv1dr7j_processed.jpeg&w=3840&q=75)
Transcribed Image Text:Part A.
Weight of skimmed milk
Isolation of Casein
Weight of dried casein
Percentage casein in skimmed milk
Part B.
1.
Identification Tests of Casein
Qualitative Tests
1.1 Biuret test.
Substance
2% glycine
2% gelatin
2% albumin
1% tyrosine
casein + distilled water
Observed Color
6.0
6.0
%
![<
1.2 Ninhydrin Test
Substance
2% glycine
2% gelatin
2% albumin
1% tyrosine
casein + distilled water
1.3 Xanthoproteic Test
Substance
2% glycine
2% gelatin
2% albumin
1% tyrosine
casein + distilled water
2. Denaturation Tests
2 of 4
Substance
Pb(NO3)2
Hg(NO3)2
Sn(IV)NO3
Observed color after heating
2.1 Heavy Metal Ion Test
Observed color before and after heating
Edit
Observed precipitate](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F531ce7ca-2de8-48a7-aa83-6b0d470c3414%2F0d2dd9bd-ec00-4016-a428-2ecf5e450b41%2Faz7lg6t_processed.png&w=3840&q=75)
Transcribed Image Text:<
1.2 Ninhydrin Test
Substance
2% glycine
2% gelatin
2% albumin
1% tyrosine
casein + distilled water
1.3 Xanthoproteic Test
Substance
2% glycine
2% gelatin
2% albumin
1% tyrosine
casein + distilled water
2. Denaturation Tests
2 of 4
Substance
Pb(NO3)2
Hg(NO3)2
Sn(IV)NO3
Observed color after heating
2.1 Heavy Metal Ion Test
Observed color before and after heating
Edit
Observed precipitate
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