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One way that metabolic pathways are mapped is by following carbons at a particular position in a molecule in what is known as isotope tracing. This can be done by synthesizing the compound with a radioactive carbon at a specific position in the molecule and then seeing which product(s) of a reaction contain that carbon atom. Through this, scientists can follow the path of that carbon and see which parts of the molecule are being chemically altered. 6. The citrate molecule drawn below has its third carbon starred (pink star). Draw the metabolites (intermediates) of all steps (one full turn) of the citric acid cycle (write the names of each enzyme over the arrows like what is shown below), starring this carbon after each step so that you can follow it through the entire pathway and see where it ends up after the final step of the citric acid cycle. (30 points) (Hint: the citrate molecule was placed in the top center of the page because you will want to draw the citric acid cycle as a loop that comes back around to the citrate molecule at the end via the citrate synthase enzyme). HS-CoA + H+ HQ COO citrate synthase -00C COO aconitase H₂O citrate

One way that metabolic pathways are mapped is by following carbons at a particular position in a molecule in what is known as isotope tracing. This can be done by synthesizing the compound with a radioactive carbon at a specific position in the molecule and then seeing which product(s) of a reaction contain that carbon atom. Through this, scientists can follow the path of that carbon and see which parts of the molecule are being chemically altered. 6. The citrate molecule drawn below has its third carbon starred (pink star). Draw the metabolites (intermediates) of all steps (one full turn) of the citric acid cycle (write the names of each enzyme over the arrows like what is shown below), starring this carbon after each step so that you can follow it through the entire pathway and see where it ends up after the final step of the citric acid cycle. (30 points) (Hint: the citrate molecule was placed in the top center of the page because you will want to draw the citric acid cycle as a loop that comes back around to the citrate molecule at the end via the citrate synthase enzyme). HS-CoA + H+ HQ COO citrate synthase -00C COO aconitase H₂O citrate

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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One way that metabolic pathways are mapped is by following carbons at a particular position in a molecule in what is known as isotope tracing. This can be done by synthesizing the compound with a radioactive carbon at a specific position in the molecule and then seeing which product(s) of a reaction contain that carbon atom. Through this, scientists can follow the path of that carbon and see which parts of the molecule are being chemically altered. 6. The citrate molecule drawn below has its third carbon starred (pink star). Draw the metabolites (intermediates) of all steps (one full turn) of the citric acid cycle (write the names of each enzyme over the arrows like what is shown below), starring this carbon after each step so that you can follow it through the entire pathway and see where it ends up after the final step of the citric acid cycle. (30 points) (Hint: the citrate molecule was placed in the top center of the page because you will want to draw the citric acid cycle as a loop that comes back around to the citrate molecule at the end via the citrate synthase enzyme). HS-CoA + H+ HQ COO citrate synthase -00C COO aconitase H₂O citrate
Transcribed Image Text:One way that metabolic pathways are mapped is by following carbons at a particular position in a molecule in what is known as isotope tracing. This can be done by synthesizing the compound with a radioactive carbon at a specific position in the molecule and then seeing which product(s) of a reaction contain that carbon atom. Through this, scientists can follow the path of that carbon and see which parts of the molecule are being chemically altered. 6. The citrate molecule drawn below has its third carbon starred (pink star). Draw the metabolites (intermediates) of all steps (one full turn) of the citric acid cycle (write the names of each enzyme over the arrows like what is shown below), starring this carbon after each step so that you can follow it through the entire pathway and see where it ends up after the final step of the citric acid cycle. (30 points) (Hint: the citrate molecule was placed in the top center of the page because you will want to draw the citric acid cycle as a loop that comes back around to the citrate molecule at the end via the citrate synthase enzyme). HS-CoA + H+ HQ COO citrate synthase -00C COO aconitase H₂O citrate
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