- Oligonucleotide primer with a mismatch Gene in -Mismatch a vector (template DNA) Vector Add DNTPS, DNA polymerase, and DNA ligase. -Mismatch The DNA is introduced into a living cell, where the mismatch is repaired. GG.C CCG CTS or A site-directed mutant is made. The DNA is repaired back to the original sequence. FIGURE 21.12 The method of site-directed mutagenesis. Genes- Traits To examine the relationship between genes and traits, researchers can alter gene sequences via site-directed mutagenesis. The altered gene is intro- duced into a living cell or organism to examine how the mutation affects the organ- ism's traits. For example, a researcher could produce a nonsense mutation in the middle of the lacY gene in the lac operon. If this site-directed mutant gene was then introduced into an E. coli bacterium that did not a have a normal copy of the lacY gene, the bacterium would be unable to use lactose. These results indicate that a functional lacY gene is necessary for bacteria to have the trait of lactose utilization.
- Oligonucleotide primer with a mismatch Gene in -Mismatch a vector (template DNA) Vector Add DNTPS, DNA polymerase, and DNA ligase. -Mismatch The DNA is introduced into a living cell, where the mismatch is repaired. GG.C CCG CTS or A site-directed mutant is made. The DNA is repaired back to the original sequence. FIGURE 21.12 The method of site-directed mutagenesis. Genes- Traits To examine the relationship between genes and traits, researchers can alter gene sequences via site-directed mutagenesis. The altered gene is intro- duced into a living cell or organism to examine how the mutation affects the organ- ism's traits. For example, a researcher could produce a nonsense mutation in the middle of the lacY gene in the lac operon. If this site-directed mutant gene was then introduced into an E. coli bacterium that did not a have a normal copy of the lacY gene, the bacterium would be unable to use lactose. These results indicate that a functional lacY gene is necessary for bacteria to have the trait of lactose utilization.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Describe three possible uses of site-directed mutagenesis.
Transcribed Image Text:- Oligonucleotide
primer with
a mismatch
Gene in
-Mismatch
a vector
(template
DNA)
Vector
Add DNTPS,
DNA polymerase,
and DNA ligase.
-Mismatch
The DNA is introduced
into a living cell, where
the mismatch is repaired.
Transcribed Image Text:GG.C
CCG
CTS
or
A site-directed mutant is made.
The DNA is repaired back
to the original sequence.
FIGURE 21.12 The method of site-directed mutagenesis.
Genes- Traits To examine the relationship between genes and traits, researchers
can alter gene sequences via site-directed mutagenesis. The altered gene is intro-
duced into a living cell or organism to examine how the mutation affects the organ-
ism's traits. For example, a researcher could produce a nonsense mutation in the
middle of the lacY gene in the lac operon. If this site-directed mutant gene was then
introduced into an E. coli bacterium that did not a have a normal copy of the lacY
gene, the bacterium would be unable to use lactose. These results indicate that a
functional lacY gene is necessary for bacteria to have the trait of lactose utilization.
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