Studies on cAMP actions in cultured cells usually involve adding to the cell culture not cAMP, but dibutyryl cAMP (see structure). Why is this structural modification necessary? How could you test the premise that di-Bu-cAMP has the same biochemical effects as cAMP?
Cyclic adenosine monophosphate (CAMP) is a secondary messenger molecule that plays an important role in many signal transduction events in biological systems Since, it is derived from ATP (adenosine triphosphate), the mechanism is conserved and ubiquitously used by various classes of organisms.
Cyclic adenosine monophosphate (CAMP) system is mainly employed by the hormones, like glucagon and epinephrine since they cannot pass through the cell membrane. The downstream effects of CAMP include activation of protein kinase A (PKA) Now, PKA gets autophosphorylated and affects the phosphorylation of downstream proteins in the pathway.
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