Ndel 183 Aatil 2617 Sspl 2501 Ehel 235 Pdml 2204 Bogl 2215 Scal 217 146/ 230 MCS lacz 2486 PUC18 2686 bp BsaXi 650 Sapi 683 AI, Pscl 806 Gsul 1784 Cfri0l 1779. Eco311 1766 1466 Eam11051 1094/ rep (PMB1) BseYl 1110 \Cail 1217 Ec136 Ctr Ecol Bam Smal Kpni Hincl Acces EcoRI Eco24 Saci Sall Xapi 455 M1C equencing prier (20 17mer 399 SG TAA AAC GAC GGC CAG TGC CAA GCT TGC ATG CCT GCA GGT CGA CTC TAG AGG ATC CC GGG TAC CGA GCT CGA ATT CGT SCATT TIG CTG ccG GTC ACG GTT CGA ACG TAC GGA CGT CCA GCT GAG ATC TCC TAG GGG CCC ATG GCT CGA GCT TAA GCA Lacz Val val Ala Leu Ala Ser Ala His Arg Cys Thr Ser Glu Leu Pro Asp Gly Pro Val Ser Ser Ser Asn The Hind Pael Sdalvel Xbal Xmil AAT CAT GGT CAT AGC TGT TTC CTG 3 TTA GTA CCA GTA TCG ACA AAG GAC S le Met Thr Met Mic reverse seuning prier (26), 17 mer Figure 2 X1 gene was inserted at the HindlII and EcoRI restriction sites of the plasmid illustrated in Figure 2. Describe the screening process to select the positive recombinants. (i) bla (Ap)
Ndel 183 Aatil 2617 Sspl 2501 Ehel 235 Pdml 2204 Bogl 2215 Scal 217 146/ 230 MCS lacz 2486 PUC18 2686 bp BsaXi 650 Sapi 683 AI, Pscl 806 Gsul 1784 Cfri0l 1779. Eco311 1766 1466 Eam11051 1094/ rep (PMB1) BseYl 1110 \Cail 1217 Ec136 Ctr Ecol Bam Smal Kpni Hincl Acces EcoRI Eco24 Saci Sall Xapi 455 M1C equencing prier (20 17mer 399 SG TAA AAC GAC GGC CAG TGC CAA GCT TGC ATG CCT GCA GGT CGA CTC TAG AGG ATC CC GGG TAC CGA GCT CGA ATT CGT SCATT TIG CTG ccG GTC ACG GTT CGA ACG TAC GGA CGT CCA GCT GAG ATC TCC TAG GGG CCC ATG GCT CGA GCT TAA GCA Lacz Val val Ala Leu Ala Ser Ala His Arg Cys Thr Ser Glu Leu Pro Asp Gly Pro Val Ser Ser Ser Asn The Hind Pael Sdalvel Xbal Xmil AAT CAT GGT CAT AGC TGT TTC CTG 3 TTA GTA CCA GTA TCG ACA AAG GAC S le Met Thr Met Mic reverse seuning prier (26), 17 mer Figure 2 X1 gene was inserted at the HindlII and EcoRI restriction sites of the plasmid illustrated in Figure 2. Describe the screening process to select the positive recombinants. (i) bla (Ap)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Question
![PUC18 (Figure 2) is a plasmid cloning vector commonly used with Escherichia coli (E. coli).
Eco01091 2674 Plol 46 BstAPI 179
Aatil 2617
Sapl 2501
Ndel 183
Ehel 235
Pdml 2204
MCS
lacz
146
Bogl 2215
Scal 2172
2486
PUC18
2686 bp
Bsaxi 659
Sapl 683
AlMI, Pscl 806
Gsul 1784
1626
Cfriol 1779
Eco311 1766
Eamt 1051 1094.
1466
rep (PMB1)
\BseYl 1110
\Cail 1217
Hincl
Ctr
Eci136
Psti
Acc65
EcoRI
Eco24
Saci
Sall
EcosBi
Xapl 455
M13puC sequencing prier (20). 17mer 399 Hindi
SG TAA AAC GAC GGC CAG TGC CAA GCT TGC ATG CCT GCA GGT CGA CTC TAG AGG ATC CC GGG TAC CGA GCT CGA ATT CGT
3'CATT TIG CTG CCG GTC ACG GTT CGA ACG TAC GGA CGT CCA GCT GAG ATC TCC TAG GGG CCC ATG GCT CGA GCT TAA GCA
Lacz -Val val Ala Leu Ala Ser Ala His Arg Cys Thr Ser Glu Leu Pro Asp Gly Pro Val Ser Ser Ser Asn Thr
Pael Sdalvel
Bam Smal Kont
Xmil
Xbal
AAT CAT GGT CAT AGC TGT TTC CTG 3
TTA GTA CCA GTA TCG ACA AAG GAC S
le Met Thr Met
MSpic reverse sequencing primer (2). 17 mer
Figure 2
X1 gene was inserted at the HindlII and EcoRI restriction sites of the plasmid illustrated
in Figure 2. Describe the screening process to select the positive recombinants.
(i)
(ii)
If X1 gene is inserted at Scal restriction site of the plasmid, what is the difficulty of the
screening process to select the potential recombinants?
bla (Ap")](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fa56c35a6-4623-4311-a953-bfaba84ab3d3%2F7f8f08d7-e9ed-4fed-8a9f-1c890676c65c%2Fwzkuque_processed.jpeg&w=3840&q=75)
Transcribed Image Text:PUC18 (Figure 2) is a plasmid cloning vector commonly used with Escherichia coli (E. coli).
Eco01091 2674 Plol 46 BstAPI 179
Aatil 2617
Sapl 2501
Ndel 183
Ehel 235
Pdml 2204
MCS
lacz
146
Bogl 2215
Scal 2172
2486
PUC18
2686 bp
Bsaxi 659
Sapl 683
AlMI, Pscl 806
Gsul 1784
1626
Cfriol 1779
Eco311 1766
Eamt 1051 1094.
1466
rep (PMB1)
\BseYl 1110
\Cail 1217
Hincl
Ctr
Eci136
Psti
Acc65
EcoRI
Eco24
Saci
Sall
EcosBi
Xapl 455
M13puC sequencing prier (20). 17mer 399 Hindi
SG TAA AAC GAC GGC CAG TGC CAA GCT TGC ATG CCT GCA GGT CGA CTC TAG AGG ATC CC GGG TAC CGA GCT CGA ATT CGT
3'CATT TIG CTG CCG GTC ACG GTT CGA ACG TAC GGA CGT CCA GCT GAG ATC TCC TAG GGG CCC ATG GCT CGA GCT TAA GCA
Lacz -Val val Ala Leu Ala Ser Ala His Arg Cys Thr Ser Glu Leu Pro Asp Gly Pro Val Ser Ser Ser Asn Thr
Pael Sdalvel
Bam Smal Kont
Xmil
Xbal
AAT CAT GGT CAT AGC TGT TTC CTG 3
TTA GTA CCA GTA TCG ACA AAG GAC S
le Met Thr Met
MSpic reverse sequencing primer (2). 17 mer
Figure 2
X1 gene was inserted at the HindlII and EcoRI restriction sites of the plasmid illustrated
in Figure 2. Describe the screening process to select the positive recombinants.
(i)
(ii)
If X1 gene is inserted at Scal restriction site of the plasmid, what is the difficulty of the
screening process to select the potential recombinants?
bla (Ap")
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