Nature of Science Ethical implications of research The use of animals in experiments may generate ethical issues. These are discussed in Appendix 3: Defining ethics and making ethical decisions on the accompanying website. Figure 2.64 4 after a fixed time, the syringe is adjusted to level the fluid in the two arms of the manometer, and the valume of O absorbed is read off on the syringe A respirometer to measure respiration rate graduated syringe- cips A and Bare closed -hypodermic needie -respirometer tube control tube (thermobarometer) water bath glass beads (same volume as sample in respirometer tube) 1 the respiring organisms gve off Co, and absorb O soda lime peliets- (Co, absorbent) -soda lime pellets (CO, absorbent 2 co, is absorbed by the soda lime U-tube manometer Any change in pressure or temperature during the experiment affects the respirometer tube and the themobarameter tube equally. 3 manometer fluid moves to the right due to the volume change caused by uptake of orygen 28 In the respirometer (Figure 2.64), explain how changes in temperature or pressure in the external environment are prevented from interfering with measurement of oxygen uptake by respiring organisms in the apparatus. 29 The experiment shown in Figure 2.64 was repeated with maggot fly larvae in tube B, first with the soda lime present and subsequently with water in place of soda lime. The volume change with soda lime was 30mm3h-1, but without soda lime it was 3mm3 h- 1. Analyse these results, explaining the significance of each value.

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Nature of Science
Ethical implications of research
The use of animals in experiments may generate ethical issues. These are discussed in
Appendix 3: Defining ethics and making ethical decisions on the accompanying website.
I Figure 2.64
4 after a fixed time, the syringe is adjusted
to level the fluid in the two arms of the
manometer, and the volume of O2
absorbed is read off on the syringe
A respirometer to
measure respiration
rate
graduated syringe
cips A and B are closed
-hypodermic
needle
respirometer tube
control tube
water bath
(thermobarometer)
glass beads (same
volume as sample in
respirometer tube)
1 the respiring organisms
give off Co, and
absorb O,
soda lime pellets-
(Co, absorbent)
-soda lime pellets
(CO, absorbent)
2 co, is absorbed by
the soda lime
U-tube manometer
Any change in pressure or temperature during
the experiment affects the respirometer tube
and the thermobarometer tube equally.
3 manometer fluid moves to the right
due to the volume change caused
by uptake of oxygen
28 In the respirometer (Figure 2.64), explain how
changes in temperature or pressure in the
external environment are prevented from
interfering with measurement of oxygen uptake
by respiring organisms in the apparatus.
29 The experiment shown in Figure 2.64 was
repeated with maggot fly larvae in tube B, first
with the soda lime present and subsequently
with water in place of soda lime. The volume
change with soda lime was 30mm3h-1, but
without soda lime it was 3mm3 h- 1. Analyse
these results, explaining the significance of each
value.
Transcribed Image Text:Nature of Science Ethical implications of research The use of animals in experiments may generate ethical issues. These are discussed in Appendix 3: Defining ethics and making ethical decisions on the accompanying website. I Figure 2.64 4 after a fixed time, the syringe is adjusted to level the fluid in the two arms of the manometer, and the volume of O2 absorbed is read off on the syringe A respirometer to measure respiration rate graduated syringe cips A and B are closed -hypodermic needle respirometer tube control tube water bath (thermobarometer) glass beads (same volume as sample in respirometer tube) 1 the respiring organisms give off Co, and absorb O, soda lime pellets- (Co, absorbent) -soda lime pellets (CO, absorbent) 2 co, is absorbed by the soda lime U-tube manometer Any change in pressure or temperature during the experiment affects the respirometer tube and the thermobarometer tube equally. 3 manometer fluid moves to the right due to the volume change caused by uptake of oxygen 28 In the respirometer (Figure 2.64), explain how changes in temperature or pressure in the external environment are prevented from interfering with measurement of oxygen uptake by respiring organisms in the apparatus. 29 The experiment shown in Figure 2.64 was repeated with maggot fly larvae in tube B, first with the soda lime present and subsequently with water in place of soda lime. The volume change with soda lime was 30mm3h-1, but without soda lime it was 3mm3 h- 1. Analyse these results, explaining the significance of each value.
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