MLS supervisor is writing an Standard Operating Procedure (SOP) for running a PC test for Hepatitis B DNA. There are several things she must include in the instructions to run the test. 1. On average, how many PCR cycles must be run to see if the test is positive or negative for HBV? 2. What would a positive test and a negative test look like? 3. Her younger sister wants to know what she did at work today. How should she explain what happens during each step of the PCR cycle.

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