Make use of the table below in answering the questions asked:Amino acid pK₁ pK₂ pK,Isoleucine 2.32 9.76Leucine2.32 9.74Lysine2.16 9.06 10.54Tyrosine2.20 9.21 10.46I1. Make sure to answer in two decimal places. What is the pl of the tripeptideILY?2. At pH 9.00, at which electrode will the ILY tripeptide be moving to?A. AnodeB. CathodeC. The tripeptide will not move to either electrodesMake sure to show a solution or explanation for the answer.

Isoelectric point is the pH at which the peptide carries a net charge of 0. The proteins are least…

Answered: Make use of the table below in…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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You have discovered a new amino acid that you name in honor of your favorite course. Twofourteeninine has an alpha-amino and an alpha-carboxylic group, and an ionizable side chain that carries an amino group. A titration curve of twofourteeninine is shown below. Net charge a P I 1 2 I 3 4 5 6 pH T 9 10 11 12 13 What are the approximate pKa values of twofourteeninine (to the nearest half-pH unit)? Check all that apply.
A 100 mL of 0.1 M amino acid at pH 1.0, whose pka for the carboxyl group is less than the pKa of the R-group, was titrated with NaOH solution. The pH was monitored, and the results were plotted on a graph, as shown below. The key points in the titration are designated A to G. 12 10 8 pH 6 4 2 0 A B 0.5 с D E F 1.5 2 2.5 Equivalents of OH 1. What is the possible identity of the amino acid? [Select] 2. What is the isoelectric point of the amino acid? [Select] 3. What is the pka corresponding to the deprotonation of the alpha-amino group? [Select] 4. Region/ point where the amino acid is predominantly present as a (-2)-charged species. [Select] 5. The effective buffering range for the amino acid in the basic region. [Select] G 3
Hemoglobin is considered to be a tetrameric complex with a 64 kDa (α β)2. When attempting to purify hemoglobin, we must first purify the α and β monomers (about 16 kDa each) to prepare the tetramer. This is formed from the dimer intermediate: 2 α + 2 β -> 2 αβ -> (α β)2. 1. The graph given represents a size-exclusion chromatogram after the refolding of the hemoglobin tetramer. This process is not 100% efficient, so we may have leftovers of dimers and monomers. In the graph given, can we label the peaks given as a tetramer, dimer, or monomer?
Draw a ceramide with a (16:1n-7) fatty acid attached
Use the table below to answer the question being asked: Protein Ovalbumin Insulin Fibrinogen y-globulin Collagen Hemoglobin (monomer) Myoglobin Isoelectric pH 4.6 5.4 5.8 6.6 6.6 7.1 7.0 Molecular weight (KDA) 45 5.7 340 160 115 16 16.7 A. Given the 7 proteins in the table, will they all be separated properly using isoelectric focusing? Why or why not? Kindly justify your answer. B. Manually sketch the appearance after visualization of a protein mixture containing the 7 proteins in the table when subjected to isoelectric focusing.
What are the charges of the following amino acids peptides at ph 14? 1. GLAVV 2. RRKKQ
1.0 0.9 Z 0.8 15. A pharmaceutical company studied the binding of three different compounds, X, Y, and Z, to a particular protein of interest. For each compound, the fractional saturation (v) was determined as a function of concentration. The results are shown in the figure. Which of the three compounds has the strongest affinity for the protein? Explain. 0.7 X 0.6 > 0.5 0.4 0.3 0.2 0.1 0.0 Y 0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 2.8 3.0 L(μM)
Regarding to ALL the 20 standard amino acids1. Are ALL the 20 standard amino acids asymmetric and chiral molecules?2. Do they contain the elements C,H,N,O,S3. do they have high boiling oils at room temperature
How is nitrogen from the atmosphere incorporated into biologically useful compounds?
What average positive charge of an amino acid side chain with pKa 10.9 at a pH value of 11.9? Answer up to two decimals. (Please type answer no write by hend)
Which of the following amino acids can interact via ionic/electrostatic interactions with lysine at physiologic pH 7.4?a. Histidineb. Asparaginec. Glutamate
The pka of the side chain of histidine is 6.00. What is the percent of protonated histidine at pH 6.4? 15.3% 28.5% 45.3% O 66.6%

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