Make a standard curve by using the values given in the table and determine the concentration of unknown sample? Tube 0,5 µg/ µl BSA 0,15 M NaCl Comassi Brillant Blue Solution Absorbance 595 nm 1 0 100 µl 900 µl 0 2 10 µl 90 µl 900 µl 0,105 3 20 µl 80 µl 900 µl 0,233 4 30 µl 70 µl 900 µl 0,41 5 40 µl 60 µl 900 µl 0,455 6 50 µl 50 µl 900 µl 0,589 Unknown Sample 10 µl 90 µl 900 µl 1,2
Make a standard curve by using the values given in the table and determine the concentration of unknown sample? Tube 0,5 µg/ µl BSA 0,15 M NaCl Comassi Brillant Blue Solution Absorbance 595 nm 1 0 100 µl 900 µl 0 2 10 µl 90 µl 900 µl 0,105 3 20 µl 80 µl 900 µl 0,233 4 30 µl 70 µl 900 µl 0,41 5 40 µl 60 µl 900 µl 0,455 6 50 µl 50 µl 900 µl 0,589 Unknown Sample 10 µl 90 µl 900 µl 1,2
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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- Make a standard curve by using the values given in the table and determine the concentration of unknown sample?
|
Tube |
0,5 µg/ µl BSA |
0,15 M NaCl |
Comassi Brillant Blue Solution |
Absorbance 595 nm |
|
1 |
0 |
100 µl |
900 µl |
0 |
|
2 |
10 µl |
90 µl |
900 µl |
0,105 |
|
3 |
20 µl |
80 µl |
900 µl |
0,233 |
|
4 |
30 µl |
70 µl |
900 µl |
0,41 |
|
5 |
40 µl |
60 µl |
900 µl |
0,455 |
|
6 |
50 µl |
50 µl |
900 µl |
0,589 |
|
Unknown Sample |
10 µl |
90 µl |
900 µl |
1,2 |
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