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Make a flow chart or a dichotomous key indicating, step by step, how you arrived at your identification: e.g. why you selected specific tests and stains, how you interpreted the results of those tests and stains, why you did nor did not accept a specific test result, etc. Bacillus subtilis Escherichia coli Klebsiella pneumoniae Micrococcus luteus Proteus mirabilis Pseudomonas aeruginosa Serratia marcescens Štaphylococcus aureus Staphylococcus epidermidis Streptococcus agalactiae

Make a flow chart or a dichotomous key indicating, step by step, how you arrived at your identification: e.g. why you selected specific tests and stains, how you interpreted the results of those tests and stains, why you did nor did not accept a specific test result, etc. Bacillus subtilis Escherichia coli Klebsiella pneumoniae Micrococcus luteus Proteus mirabilis Pseudomonas aeruginosa Serratia marcescens Štaphylococcus aureus Staphylococcus epidermidis Streptococcus agalactiae

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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The Unknown specimen for identification is Staphylococcus epidermidis
### Educational Resource: Creating a Flow Chart/Dichotomous Key for Bacterial Identification #### Task Make a flow chart or a dichotomous key indicating, step by step, how you arrived at your identification. Consider the following: - Why you selected specific tests and stains. - How you interpreted the results of those tests and stains. - Why you did or did not accept a specific test result, etc. #### Bacterial Species for Identification: 1. **Bacillus subtilis** 2. **Escherichia coli** 3. **Klebsiella pneumoniae** 4. **Micrococcus luteus** 5. **Proteus mirabilis** 6. **Pseudomonas aeruginosa** 7. **Serratia marcescens** 8. **Staphylococcus aureus** 9. **Staphylococcus epidermidis** 10. **Streptococcus agalactiae** ### Steps to Create a Flow Chart or Dichotomous Key 1. **Select Specific Tests and Stains**: Choose tests based on the likely characteristics of the bacteria such as Gram staining, oxygen requirement, fermentation abilities, enzyme production (e.g., catalase, oxidase, urease), and motility. 2. **Perform Tests and Record Results**: Conduct each test carefully. Record whether bacteria are Gram-positive or Gram-negative, the shape, and grouping of cells, and results from biochemical tests. 3. **Interpret Results Step-by-Step**: Compare the test results to known bacterial characteristics to narrow down the possibilities. 4. **Flow Chart/Dichotomous Key**: - Start with broad categories (e.g., Gram-positive vs. Gram-negative). - Move to more specific tests (e.g., catalase test for Gram-positive cocci, oxidase test for Gram-negative rods). - Continue to progressively more specific tests until a single species can be identified. ### Example Flow Chart Structure 1. **Gram Stain Test**: - Gram-positive: Proceed to catalase test. - Gram-negative: Proceed to oxidase test. 2. **Catalase Test (for Gram-positive)**: - Catalase positive: Staphylococcus spp. (Proceed to coagulase test) - Catalase negative: Streptococcus spp. (Identify hemolysis pattern on blood agar) 3. **Oxidase Test (for Gram-negative)**: - Oxidase positive:
Transcribed Image Text:### Educational Resource: Creating a Flow Chart/Dichotomous Key for Bacterial Identification #### Task Make a flow chart or a dichotomous key indicating, step by step, how you arrived at your identification. Consider the following: - Why you selected specific tests and stains. - How you interpreted the results of those tests and stains. - Why you did or did not accept a specific test result, etc. #### Bacterial Species for Identification: 1. **Bacillus subtilis** 2. **Escherichia coli** 3. **Klebsiella pneumoniae** 4. **Micrococcus luteus** 5. **Proteus mirabilis** 6. **Pseudomonas aeruginosa** 7. **Serratia marcescens** 8. **Staphylococcus aureus** 9. **Staphylococcus epidermidis** 10. **Streptococcus agalactiae** ### Steps to Create a Flow Chart or Dichotomous Key 1. **Select Specific Tests and Stains**: Choose tests based on the likely characteristics of the bacteria such as Gram staining, oxygen requirement, fermentation abilities, enzyme production (e.g., catalase, oxidase, urease), and motility. 2. **Perform Tests and Record Results**: Conduct each test carefully. Record whether bacteria are Gram-positive or Gram-negative, the shape, and grouping of cells, and results from biochemical tests. 3. **Interpret Results Step-by-Step**: Compare the test results to known bacterial characteristics to narrow down the possibilities. 4. **Flow Chart/Dichotomous Key**: - Start with broad categories (e.g., Gram-positive vs. Gram-negative). - Move to more specific tests (e.g., catalase test for Gram-positive cocci, oxidase test for Gram-negative rods). - Continue to progressively more specific tests until a single species can be identified. ### Example Flow Chart Structure 1. **Gram Stain Test**: - Gram-positive: Proceed to catalase test. - Gram-negative: Proceed to oxidase test. 2. **Catalase Test (for Gram-positive)**: - Catalase positive: Staphylococcus spp. (Proceed to coagulase test) - Catalase negative: Streptococcus spp. (Identify hemolysis pattern on blood agar) 3. **Oxidase Test (for Gram-negative)**: - Oxidase positive:
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