Look at the diagram below of a ten-fold serial dilution. Serial Dilution 9 mL stock solution 1 mL stock solution & 9 mL saline 9 mL saline 9 mL saline 9 ml saline solution 9 ml saline solution solution solution 1 mL D2 solution to D3 1 mL D3 solution to D4 1 mL D4 solution to DS 1 mL D5 solution to waste D1 D5 D2 D3 D4 D6 In this diagram, D1 is the original solution. For a ten-fold serial dilution, first add 9 mL of solvent (eg. Saline) to 5 new tubes marked D2-D6. Take 1 mL of D1 solution, add to tube D2 and mix. Take 1 mL of D2, add to D3, and mix. Take 1 mL of D3, add to D4, and mix. Take 1 mL of D4, add to D5, and mix. Take 1 mL of D5, add to D6, and mix. Problem 2. If tube D1 has 100% concentration, then what is the % concentration of tube D2? tube D3? tube D4? tube D5?

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Look at the diagram below of a ten-fold serial dilution.
Serial Dilution
1 mL stock
9 mL stock solution &
9 ml saline
9 mL saline 9 mL saline 9 mL saline 9 mL saline
solution
solution
solution
solution
solution
1 mL
D2
solution
to D3
1 mL
D3
solution
to D4
1 mL
D4
solution
to D5
1 mL
D5
solution
to waste
D1
D2
D3
D4
D5
D6
In this diagram, D1 is the original solution. For a ten-fold
serial dilution, first add 9 mL of solvent (eg. Saline) to 5 new
tubes marked D2-D6. Take 1 mL of D1 solution, add to tube
D2 and mix. Take 1 mL of D2, add to D3, and mix. Take 1
mL of D3, add to D4, and mix. Take 1 mL of D4, add to D5,
and mix. Take 1 mL of D5, add to D6, and mix.
Problem 2. If tube D1 has 100% concentration, then what is
the % concentration of tube D2?
tube D3?
tube D4?
tube D5?
Transcribed Image Text:Look at the diagram below of a ten-fold serial dilution. Serial Dilution 1 mL stock 9 mL stock solution & 9 ml saline 9 mL saline 9 mL saline 9 mL saline 9 mL saline solution solution solution solution solution 1 mL D2 solution to D3 1 mL D3 solution to D4 1 mL D4 solution to D5 1 mL D5 solution to waste D1 D2 D3 D4 D5 D6 In this diagram, D1 is the original solution. For a ten-fold serial dilution, first add 9 mL of solvent (eg. Saline) to 5 new tubes marked D2-D6. Take 1 mL of D1 solution, add to tube D2 and mix. Take 1 mL of D2, add to D3, and mix. Take 1 mL of D3, add to D4, and mix. Take 1 mL of D4, add to D5, and mix. Take 1 mL of D5, add to D6, and mix. Problem 2. If tube D1 has 100% concentration, then what is the % concentration of tube D2? tube D3? tube D4? tube D5?
1 ml
1 ml
1 ml
1 ml
A
B
D
1 ml
1 ml
1 ml
1 ml
STOCK SOLUTION
9 ml
9 ml
9 ml
9 ml
9 ml =distilled water
1 ml= stock solution
1/10 th
dilution
1/100 th
1/1000 th
1/10000 th
dilution
dilution
dilution
or .1 or
or .0001
or 10-4
www.biologyexams4u.com
or .01 or
or .001 or
10-1
10-2
10-3
dilution
dilution
dilution
dilution
Transcribed Image Text:1 ml 1 ml 1 ml 1 ml A B D 1 ml 1 ml 1 ml 1 ml STOCK SOLUTION 9 ml 9 ml 9 ml 9 ml 9 ml =distilled water 1 ml= stock solution 1/10 th dilution 1/100 th 1/1000 th 1/10000 th dilution dilution dilution or .1 or or .0001 or 10-4 www.biologyexams4u.com or .01 or or .001 or 10-1 10-2 10-3 dilution dilution dilution dilution
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