Look at the diagram below of a ten-fold serial dilution. Serial Dilution 9 mL stock solution 1 mL stock solution & 9 mL saline 9 mL saline 9 mL saline 9 ml saline solution 9 ml saline solution solution solution 1 mL D2 solution to D3 1 mL D3 solution to D4 1 mL D4 solution to DS 1 mL D5 solution to waste D1 D5 D2 D3 D4 D6 In this diagram, D1 is the original solution. For a ten-fold serial dilution, first add 9 mL of solvent (eg. Saline) to 5 new tubes marked D2-D6. Take 1 mL of D1 solution, add to tube D2 and mix. Take 1 mL of D2, add to D3, and mix. Take 1 mL of D3, add to D4, and mix. Take 1 mL of D4, add to D5, and mix. Take 1 mL of D5, add to D6, and mix. Problem 2. If tube D1 has 100% concentration, then what is the % concentration of tube D2? tube D3? tube D4? tube D5?

Chemistry for Engineering Students
4th Edition
ISBN:9781337398909
Author:Lawrence S. Brown, Tom Holme
Publisher:Lawrence S. Brown, Tom Holme
Chapter3: Molecules, Moles, And Chemical Equations
Section: Chapter Questions
Problem 3.66PAE
icon
Related questions
Question
Look at the diagram below of a ten-fold serial dilution.
Serial Dilution
1 mL stock
9 mL stock solution &
9 ml saline
9 mL saline 9 mL saline 9 mL saline 9 mL saline
solution
solution
solution
solution
solution
1 mL
D2
solution
to D3
1 mL
D3
solution
to D4
1 mL
D4
solution
to D5
1 mL
D5
solution
to waste
D1
D2
D3
D4
D5
D6
In this diagram, D1 is the original solution. For a ten-fold
serial dilution, first add 9 mL of solvent (eg. Saline) to 5 new
tubes marked D2-D6. Take 1 mL of D1 solution, add to tube
D2 and mix. Take 1 mL of D2, add to D3, and mix. Take 1
mL of D3, add to D4, and mix. Take 1 mL of D4, add to D5,
and mix. Take 1 mL of D5, add to D6, and mix.
Problem 2. If tube D1 has 100% concentration, then what is
the % concentration of tube D2?
tube D3?
tube D4?
tube D5?
Transcribed Image Text:Look at the diagram below of a ten-fold serial dilution. Serial Dilution 1 mL stock 9 mL stock solution & 9 ml saline 9 mL saline 9 mL saline 9 mL saline 9 mL saline solution solution solution solution solution 1 mL D2 solution to D3 1 mL D3 solution to D4 1 mL D4 solution to D5 1 mL D5 solution to waste D1 D2 D3 D4 D5 D6 In this diagram, D1 is the original solution. For a ten-fold serial dilution, first add 9 mL of solvent (eg. Saline) to 5 new tubes marked D2-D6. Take 1 mL of D1 solution, add to tube D2 and mix. Take 1 mL of D2, add to D3, and mix. Take 1 mL of D3, add to D4, and mix. Take 1 mL of D4, add to D5, and mix. Take 1 mL of D5, add to D6, and mix. Problem 2. If tube D1 has 100% concentration, then what is the % concentration of tube D2? tube D3? tube D4? tube D5?
1 ml
1 ml
1 ml
1 ml
A
B
D
1 ml
1 ml
1 ml
1 ml
STOCK SOLUTION
9 ml
9 ml
9 ml
9 ml
9 ml =distilled water
1 ml= stock solution
1/10 th
dilution
1/100 th
1/1000 th
1/10000 th
dilution
dilution
dilution
or .1 or
or .0001
or 10-4
www.biologyexams4u.com
or .01 or
or .001 or
10-1
10-2
10-3
dilution
dilution
dilution
dilution
Transcribed Image Text:1 ml 1 ml 1 ml 1 ml A B D 1 ml 1 ml 1 ml 1 ml STOCK SOLUTION 9 ml 9 ml 9 ml 9 ml 9 ml =distilled water 1 ml= stock solution 1/10 th dilution 1/100 th 1/1000 th 1/10000 th dilution dilution dilution or .1 or or .0001 or 10-4 www.biologyexams4u.com or .01 or or .001 or 10-1 10-2 10-3 dilution dilution dilution dilution
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 5 steps

Blurred answer
Knowledge Booster
Tools in Analytical Chemistry
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.
Recommended textbooks for you
Chemistry for Engineering Students
Chemistry for Engineering Students
Chemistry
ISBN:
9781337398909
Author:
Lawrence S. Brown, Tom Holme
Publisher:
Cengage Learning
Introductory Chemistry For Today
Introductory Chemistry For Today
Chemistry
ISBN:
9781285644561
Author:
Seager
Publisher:
Cengage
Introductory Chemistry: A Foundation
Introductory Chemistry: A Foundation
Chemistry
ISBN:
9781285199030
Author:
Steven S. Zumdahl, Donald J. DeCoste
Publisher:
Cengage Learning
Chemistry & Chemical Reactivity
Chemistry & Chemical Reactivity
Chemistry
ISBN:
9781337399074
Author:
John C. Kotz, Paul M. Treichel, John Townsend, David Treichel
Publisher:
Cengage Learning
Chemistry: The Molecular Science
Chemistry: The Molecular Science
Chemistry
ISBN:
9781285199047
Author:
John W. Moore, Conrad L. Stanitski
Publisher:
Cengage Learning
Introductory Chemistry: A Foundation
Introductory Chemistry: A Foundation
Chemistry
ISBN:
9781337399425
Author:
Steven S. Zumdahl, Donald J. DeCoste
Publisher:
Cengage Learning