Ligand L binds to a binding protein with a Ka of 400 nM. Calculate the ligand concentration based on the fraction of binding sites occupied by the ligand (Y). Express each answer to two significant figures. Calculate the ligand concentration when Y is 0.25. [L] = µM Calculate the ligand concentration when Y is 0.60. [L] = µM Calculate the ligand concentration when Y is 0.95.

Ligand L binds to a binding protein with a Ka of 400 nM. Calculate the ligand concentration based on the fraction of binding sites occupied by the ligand (Y). Express each answer to two significant figures. Calculate the ligand concentration when Y is 0.25. [L] = µM Calculate the ligand concentration when Y is 0.60. [L] = µM Calculate the ligand concentration when Y is 0.95.

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Question

Ligand L binds to a binding protein with a Ka of 400 nM. Calculate the ligand concentration based on the fraction of binding
sites occupied by the ligand (Y). Express each answer to two significant figures.
Calculate the ligand concentration when Y is 0.25.
[L] =
µM
Calculate the ligand concentration when Y is 0.60.
[L] =
µM
Calculate the ligand concentration when Y is 0.95.
[L]
µM

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