Answer the question It is time to transfect the construct of yourchoice into mouse cells. What type ofmouse cells should be transfected? What isthe rationale to use that particular cell type?

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Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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It is time to transfect the construct of your
choice into mouse cells. What type of
mouse cells should be transfected? What is
the rationale to use that particular cell type?

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Step 1

Downstream analysis of gene constructs in mammalian system is essential to understand their effects at genetic level. Embryonic cells have an advantage over other cell types due to their indefinite proliferation and renewal and its capacity to differentiate into all germ lines, namely ectoderm, mesoderm and endoderm. Moreover, these cells can easily be modified genetically using a variety of techniques like liposomes, chemicals, viruses and electroporation.

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