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- 2a) With the aid of diagrams, describe the critical role of restriction enzymes in genetic engineering? b) Write short notes on any two characteristics of a vectorDescribe briefly the perks, disadvantages and use of 16s rRNA genes in taxonomic level of classification of bacteria. Cite the claims to be discussed, only here: https://journals.asm.org/doi/full/10.1128/CMR.17.4.840-862.2004 Create a review format of the task.Differentiate between TWO of the following pairs: Genetic and a restriction map Southern and colony blotting Polymerase chain reaction (PCR) and cloning
- Why do we want to genetically manipulate bacterial? how can we genetically manipulate bacteria? How can we tell if we have successful transaction?How can RNA sequencing (RNA-Seq) and Mirccorays in Transcriptome analysis find disease genes? Please lay out the answer in steps and explain them.What are the reagents used for Reverse Transcriptase PCR (RT-PCR) and Reverse Transcriptase–Real time–PCR (RT-qPCR)?