Is it possible to perform DNA profiling with SNPs instead of SSRs as DNA markers, but in general you would need to examine more SNP markers that the 13 SSRr used in the CODIS database to be sure of a match. Explain why.

Q: How real time RT-PCR can be used in detecting SARS-nCov2 virus? Please explain at your own words.

A: Reverse transcription- Polymerase chain reaction (RT-PCR) is a technique in which the RNA is…

Q: When a company changes from the LAL assay to the Recombinant Factor C assay, they must perform a…

A: LAL or Limulus amebocyte lysate assay is a technique used to assess and quantify the presence of…

Q: Which genetic application would you consider as the most significant during this time of pandemic?…

A: Introduction :- Genetic engineering may be employed on a wide range of plants, animals, and microbes…

Q: test, four target gene are used: viral RdRp, viral S protein, viral N protein, and human RPP30. On a…

A: In the PCR test, the primer is used to determine the specific sequence present in the sample.

Q: In Colony Polymerase Chain Rxn (PCR), what primers are necessary to identify that the transformants…

A: In Colony Polymerase Chain Reaction (PCR), specific primers are used to identify transformants…

Q: What should be the ideal characteristics of a base for suppositories dosage form?  Please briefly…

A: Suppository It is administered to produce either systemic or local effect, in solid dosage form.…

Q: Why does it make sense to look for ARGs and not antibiotic-resistant organisms themselves?

A: Antibiotic resistance genes (ARGs) are segments of DNA that encode for proteins or enzymes that…

Q: Differentiate Invitrogen pCR® II-TOPO® Vector from pBR322 Vector.

A: Molecular cloning : Production of genetically identical copies of a cell or its genetic material is…

Q: What do you need to pay attention to when designing SNP specific primers for the detection of gene…

A: When we designing SNP specific primers for the detection of gene sequences pay attention on…

Q: When gel electrophoresis is undertaken, one well always contains DNA-marker molecules. The purpose…

A: The objective of the question is to understand the purpose of DNA-marker molecules in a gel…

Q: When you go to the lab and you carry out the reaction several times, you realize that you made the…

A: The polymerase chain reaction is a technique in molecular biology that is used to amplify the target…

Q: Briefly explain the rationales of adding Bisacridine A which can affect DNA stability in polymerase…

A: PCR is used to enhance genes associated with patients' DNA genetic disorders. It has practical uses…

Q: A newly developed qPCR has an efficiency of 75%, and each cycle is pretty consistent. In this qPCR,…

A: Probe is a single-stranded sequence of DNA or RNA used to search for its complementary sequence in a…

Q: The following figure shows the FBI-style analysis of the genomic DNA of 10 people (1-10), and also…

A: Chromosomes are carrier of deoxyribonucleic acid (DNA). DNA is the genetic material. Simple sequence…

Q: Currently I am working with qPCR quantification specific for Lactobacillus bacteria. I found in some…

A: The melting curves are generated when the DNA is heated along with the fluorescent DNA-binding dye.…

Q: For your Southern Blot, every band showed a reaction with the probe.Describe what happened and how…

A: Southern Blot is a laboratory technique that is used to detect specific DNA molecules from various…

Q: ii

A: Antibiotic help in the selection of transformed bacteria against non transformed bacteria.

Q: a. It is possible to perform DNA fingerprinting withSNPs instead of SSRs as DNA markers, but…

A: DNA fingerprinting is a chemical test used to determine the genetic makeup of a person. It involves…

Q: Coomassie dye is the equivalent of what component used in analysis of DNA by agarose gel…

A: Western blot helps to identify specific proteins from a mixture of proteins and this step follows…

Q: Sulfatase 1 (Sulf1) is a lizard blastema marker   Identify a region of the Gekko japonicus Sulf1…

A: I used the NCBI Primer/Nucleotide Blast tool to compare the protein sequences of Gekko japonicus…

Q: What is DNA barcoding, and what types of cases use this profiling method?

A: DNA barcoding is an instrument to recognize various species. Mitochondrial DNA inheritance ( mtDNA)…

Q: when creating a transgenic animal Explain why you need to culture the E. coli bacteria on agar that…

A: Transgenic refers to the artificial introduction of one or even multiple DNA sequences from another…

Q: cite one reason why the RT-PCR test for COVID-19 detection may give a false negative result. assume…

A: RT PCR is reverse transcptase polymerase chain reaction used to determine the presence of virus in…

Q: 3 types of common coronavirus tests (RT-PCR for viral RNA with Taqman detection, lateral flow…

A: Coronavirus has spread like a wildfire and the disease caused by it has turned into a global…

Q: Suppose that 25,000 individuals were tested for COVID-19 using rapid diagnostic test (RDT). Of…

A: Positive predictive value means that the subjects who were tested are actually positive it is…

Q: Here you see the gel electrophoresis of PCR reactions of the pMCT118 VNTR locus of 4 individuals…

A: Answer pMCT118 VNTR  locus has multiple repeats that can be analyzed by Polymerase chain reaction…

Q: 3 different pcr tubes were obtained each tube containing a variation of hemoglobin E. tube A is…

A: Gel electrophoresis is the migration of DNA or protein molecules on the electric field. This…

Q: The main reason/reasons that motivated the genetic engineers to use E. coli in the production of…

A: Ans: Genetic engineering: It is the process in which the foreign gene is cloned into host cells for…

Q: . The concept of (CODIS, multiplexing) involves simultaneous detection of more than one DNA marker.

A: This is called Multiplexing.

Q: E. coli cells are simultaneously infected with two strains of phage λ. One strain has a mutant host…

A: A gene is a stretch of nucleotides present in the DNA molecule. It encodes information for the…

Q: The gel below shows results for the bitter tasting SNP analysis. Analyze the results and annotate…

A: PCR or polymerase chain reaction is a technique that is used to amplify the desired segment of DNA…

Q: Some compounds are "frequent hitters" or pan-assay interferences (PAINS). Does having a really…

A: PAINs (Pan-Assay Interference Compounds) are small molecule compounds that have been found to…

Q: Please describe the role of each buffers (P1, P2, N3, PE, and EB) and RNAase A and Lyse blue during…

A: Role of P1 buffer - This buffer usually consists of a basic pH Tris buffer with EDTA…

Q: What kind of DNA is actually used to make the spots for analysis for the Vicrochip DNA microassay?

A: DNA (deoxyribonucleic acid) microarray or DNA chip is a technique that is used to analyze the…

Q: Can you think and answer how a reporter enzyme can be used to monitor transformation of host cells…

A: An example of a reporter gene is the Lac Z gene, this gene encodes for fluorescent proteins in the…

Q: Why would you look for ARGs in a farming context And why specifically in the waste manure produced…

A: In the context of microbiology and genetics, ARG stands for antibiotic resistance gene. These are…

Q: 3.) A number of publications explain an expanding knowledge on the genetics and genomics of…

A: SARS-CoV-2: SARS-CoV-2 is the virus responsible for causing the COVID-19 pandemic that began in…

Q: What is the prevalence of COVID-19 in this population? A. 0.5% B. 1.5% C. 2.5% D. 3% 45. What…

A: Given  Total people = 25000  No. of true positive result (TP) =  340  no. Of false positive result…

Q: The vector below was used in the cloning process. Eco01091 2674 BstAPI 179 Aatll 2617 Ndel 183 Sspl…

A: Answer- pUC18/19 is an artificial plasmid  created. Which has antibiotics resistance gene…

Q: Explain your results when observations as stated below were obtained after the plasmids were…

A: DNA is a polymer composed of two polynucleotide chains that coil around each other to form a double…

Genomic studies

Genomic studies (genomics) is the study of the whole genome of an organism. Using bioinformatics and high-performance computing, genomic researchers examined massive amounts of deoxyribonucleic acid (DNA)-sequence data to discover variations or mutations that affect drug response, health, or disease. Genomics is a much younger discipline than genetics, having only emerged in the last few decades as a result of technological advancements in DNA sequencing and computational biology.