Initial rates of an enzyme -catalysed reaction at different substrate concentrations are listed in Table 2.1 s, mol/L 0.0041 0.00095 0.00052 0.000103 0.000049 v, mol/(L. min) x 10° 177 173 125 106 80 0.0000106 0.0000051 a) Evaluate K and vmax by a Lineweaver-Burk plot b) Using the Eadie-Hofstee plot, evaluate K and Vmax c) Using theHanes-Woolf plot, evaluate K and Vmax d) Comment on the accuracy of the three methods 67 43 E

Chemistry
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Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
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Chapter1: Chemical Foundations
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Initial rates of an enzyme -catalysed reaction at different substrate concentrations are listed in Table 2.1
s, mol/L
0.0041
0.00095
0.00052
0.000103
0.000049
v, mol/(L. min) x 10°
177
173
125
106
80
0.0000106
0.0000051
a) Evaluate K and vmax by a Lineweaver-Burk plot
b) Using the Eadie-Hofstee plot, evaluate K and Vmax
c) Using theHanes-Woolf plot, evaluate K and Vmax
d) Comment on the accuracy of the three methods
67
43
E
Transcribed Image Text:Initial rates of an enzyme -catalysed reaction at different substrate concentrations are listed in Table 2.1 s, mol/L 0.0041 0.00095 0.00052 0.000103 0.000049 v, mol/(L. min) x 10° 177 173 125 106 80 0.0000106 0.0000051 a) Evaluate K and vmax by a Lineweaver-Burk plot b) Using the Eadie-Hofstee plot, evaluate K and Vmax c) Using theHanes-Woolf plot, evaluate K and Vmax d) Comment on the accuracy of the three methods 67 43 E
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