In what order does initiation of DNA replication proceed (from 1 = first to 4 = last)? DNA polymerase adds nucleotides DNA unwinds at the origin of replication Primase synthesizes RNA primers Helicase opens up replication forks, which extend in both directions

In what order does initiation of DNA replication proceed (from 1 = first to 4 = last)? DNA polymerase adds nucleotides DNA unwinds at the origin of replication Primase synthesizes RNA primers Helicase opens up replication forks, which extend in both directions

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Description: In what order does initiation of DNA replication proceed (from 1 = first to 4 = last)? DNA polymerase adds nucleotides DNA unwinds at the origin

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter8: The Structure, Replication, And Chromosomal Organization Of Dna

Section: Chapter Questions

Problem 23QP: How does DNA replication occur in a precise manner to ensure that identical genetic information is...

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Why is DNA replication called semiconservative?
Match the enzymes involved in DNA replication with their function. Primase [ Choose ] [ Choose] Synthesizes short RNA segment to initiate new DNA strand Helicase Main enzyme that extends RNA primer by adding DNA nucleotides to it Stabilizes single-stranded DNA Relieves over-winding of DNA ahead of the replication fork Removes RNA primers preceding Okazaki fragment and replaces RNA nucleotides with DNA nucleotides Single-stranded binding proteins Unwinds DNA helix Synthesizes the ends of the linear chromosome Seals nicks between adjacent DNA segments DNA polymerase III [ Choose ] DNA polymerasel [ Choose ] DNA Ligase [ Choose ] Topoisomerase [ Choose ]
DNA polymerases are processive, which means that they remain tightly associated with the template strand while moving rapidly and adding nucleotides to the growing daughter stand. Which piece of the replication machinery accounts for this characteristic? Helicase Sliding Clamp Single Stranded Binding Protein Primase
Define DNA replication/synthesis and semiconservative replication. In addition, describe and/or define the role(s) of each of the following in the process of DNA replication/synthesis: DNA template strand, 5’ and 3’ ends, DNA helicase, DNA polymerase, single-strand binding proteins, topoisomerase, primase, Okazaki fragments, leading strand and lagging strand.
During DNA replication, the function of RNA primers is to Group of answer choices serve as a binding site for DNA ligase separate the two strands of the double helix to open replication "bubbles" serve as starting points for DNA strand elongation by DNA polymerase in the 3' - 5' direction prevent new-separated strands of DNA from rejoining serve as starting points for DNA strand elongation in the 5' - 3' direction by DNA polymerase
What is the function of DNA primase in DNA replication? to insert new bases during elongation, using the parent strand as a template to anneal (splice) smaller fragments of DNA to create one continuous strand to create an RNA primer to initiate DNA replication to create replication bubbles by unravelling the parental strands of DNA all of the above
What is the difference between the leading strand and the lagging strand in DNA replication? There are different DNA polymerases involved in elongation of the leading strand and the lagging strand. The leading strand is synthesized continuously in the 5' → 3' direction, while the lagging strand is synthesized discontinuously in the 5' → 3' direction. The leading strand requires an RNA primer, whereas the lagging strand does not. The leading strand is synthesized in the 3' → 5' direction in a discontinuous fashion, while the lagging strand is synthesized in the 5' → 3' direction in a continuous fashion.
Place the following steps of DNA replication in order (from left to right) from the beginning to the end of the process. Reset Help priming of DNA synthesis with RNA primer removal of primers by DNA polymerase I exonuclease elongation of RNA primers by DNA polymerase III opening and stabilizing the DNA strands joining of Okazaki fragments by DNA ligase Beginning of replication End of replication
Match the following descriptions with the enzymes involved in DNA replication. Adds an RNA primer to begin elongation Removes the RNA primer from the beginning of the newly constructed strands Splices lagging strand segments Cleaves the rung of the DNA double helix ladder Description: Answer Answer Answer Answer Enzyme: Helicase DNA Polymerase Primase DNA Ligase
Arrange the events of replication chronologically from A (first) to G (last event): -Sealing of phosphodiester linkage -Replacement of RNA primer with new DNA strand -Unwinding of the double helix -Addition of short RNA primers -Recognition of origin of replication -Synthesis of complementary DNA strand at the leading strand of the DNA -Excision of short RNA primers
DNA replication occurs by adding (Note: NTPS = nucleotide triphosphates; dNTPs = deoxynucleotide triphosphates) DNTPS to the 3' end of the template strand NTPS to the 3' end of the daughter strand DNTPS to the 3' end of the daughter strand DNTPS to the 5' end of the template strand NTPS to the 5' end of the daughter strand
The figure below depicts various elements of the eukaryotic replication machinery in action. Enter the name for the protein depicted by each box. Box A Box B Box C Box D Box E Box F DNA polymerase on lagging strand (just finishing an Okazaki fragment) F Maintains polymerase association with DNA Enzyme extends separation of DNA strands Synthesizes RNA fragments that hybridize to DNA Relaxes supercoiled DNA ahead of replication fork Maintains DNA is single stranded state Promotes binding of processivity factors to DNA Newly synthesized strand pocoar Leading-strand template A New Okazaki fragment RNA primer E Lagging-strand template DNA polymerase on leading strand B C D Saaragon - Next Okazaki fragment will start here Parental DNA helix