In the synthesis of fluorescein reaction after the reaction mixture is heated and kept at a temperature of 160 – 180˚C you then treat the product with a solution of a strong base (1.0 M sodium hydroxide) followed by a strong acid (1.0 M hydrochloric acid). What is the purpose of these steps?

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Chapter1: Chemical Foundations
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In the synthesis of fluorescein reaction after the reaction mixture is heated and kept at a temperature of 160 – 180˚C you then treat the product with a solution of a strong base (1.0 M sodium hydroxide) followed by a strong acid (1.0 M hydrochloric acid). What is the purpose of these steps?

 

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