Homework Help is Here – Start Your Trial Now!
Science
Biology
. In the experiment shown below, fibroblasts obtained from a normal subject (closed symbols) or from a patient homozygous for familial hypercholester- olemia (FH Homozygote) (open symbols) were grown in monolayer cultures. At time zero, the medium was replaced with fresh medium depleted of lipoproteins, and HMG-COA reductase activity was measured in extracts prepared at the indicated times (panel a). Twenty-four hours after addition of the lipoprotein- deficient medium, human LDL was added to the cells at the indicated levels, and HMG-COA reductase activity was measured at the indicated time. AFTER REMOVAL OF LIPOPROTEINS AFTER ADDITION OF LDL 200 FH Homozygote- LDL 100 None - Normal 2 ug/ml 10 20 µg/ml 8. 16 24 32 8. 12 Hours Hours (a) (b) Based on your understanding of HMG-CoA reductase regulation, explain the following results: (a) When cultured in the presence of L.DL, normal cells showed low activity of HMG-COA reductase. After removal of lipopoteins, including LDIL, HMG-COA reductase activities increased some 50- to 100-fold in normal cells (panel a). This high level of enzyme activity was rapidly suppressed upon addition of L.DL back to normal cells (panel b). (b) Cells from FH individuals showed high levels of reductase activity, whether cultured in the presence or absence of LDL. HMG COA Reductase Activity (pmoles/min/mg) LILLI LII

. In the experiment shown below, fibroblasts obtained from a normal subject (closed symbols) or from a patient homozygous for familial hypercholester- olemia (FH Homozygote) (open symbols) were grown in monolayer cultures. At time zero, the medium was replaced with fresh medium depleted of lipoproteins, and HMG-COA reductase activity was measured in extracts prepared at the indicated times (panel a). Twenty-four hours after addition of the lipoprotein- deficient medium, human LDL was added to the cells at the indicated levels, and HMG-COA reductase activity was measured at the indicated time. AFTER REMOVAL OF LIPOPROTEINS AFTER ADDITION OF LDL 200 FH Homozygote- LDL 100 None - Normal 2 ug/ml 10 20 µg/ml 8. 16 24 32 8. 12 Hours Hours (a) (b) Based on your understanding of HMG-CoA reductase regulation, explain the following results: (a) When cultured in the presence of L.DL, normal cells showed low activity of HMG-COA reductase. After removal of lipopoteins, including LDIL, HMG-COA reductase activities increased some 50- to 100-fold in normal cells (panel a). This high level of enzyme activity was rapidly suppressed upon addition of L.DL back to normal cells (panel b). (b) Cells from FH individuals showed high levels of reductase activity, whether cultured in the presence or absence of LDL. HMG COA Reductase Activity (pmoles/min/mg) LILLI LII

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
See similar textbooks
icon
Related questions
Question
. In the experiment shown below, fibroblasts obtained from a normal subject (closed symbols) or from a patient homozygous for familial hypercholester- olemia (FH Homozygote) (open symbols) were grown in monolayer cultures. At time zero, the medium was replaced with fresh medium depleted of lipoproteins, and HMG-COA reductase activity was measured in extracts prepared at the indicated times (panel a). Twenty-four hours after addition of the lipoprotein- deficient medium, human LDL was added to the cells at the indicated levels, and HMG-COA reductase activity was measured at the indicated time.
Transcribed Image Text:. In the experiment shown below, fibroblasts obtained from a normal subject (closed symbols) or from a patient homozygous for familial hypercholester- olemia (FH Homozygote) (open symbols) were grown in monolayer cultures. At time zero, the medium was replaced with fresh medium depleted of lipoproteins, and HMG-COA reductase activity was measured in extracts prepared at the indicated times (panel a). Twenty-four hours after addition of the lipoprotein- deficient medium, human LDL was added to the cells at the indicated levels, and HMG-COA reductase activity was measured at the indicated time.
AFTER REMOVAL OF LIPOPROTEINS AFTER ADDITION OF LDL 200 FH Homozygote- LDL 100 None - Normal 2 ug/ml 10 20 µg/ml 8. 16 24 32 8. 12 Hours Hours (a) (b) Based on your understanding of HMG-CoA reductase regulation, explain the following results: (a) When cultured in the presence of L.DL, normal cells showed low activity of HMG-COA reductase. After removal of lipopoteins, including LDIL, HMG-COA reductase activities increased some 50- to 100-fold in normal cells (panel a). This high level of enzyme activity was rapidly suppressed upon addition of L.DL back to normal cells (panel b). (b) Cells from FH individuals showed high levels of reductase activity, whether cultured in the presence or absence of LDL. HMG COA Reductase Activity (pmoles/min/mg) LILLI LII
Transcribed Image Text:AFTER REMOVAL OF LIPOPROTEINS AFTER ADDITION OF LDL 200 FH Homozygote- LDL 100 None - Normal 2 ug/ml 10 20 µg/ml 8. 16 24 32 8. 12 Hours Hours (a) (b) Based on your understanding of HMG-CoA reductase regulation, explain the following results: (a) When cultured in the presence of L.DL, normal cells showed low activity of HMG-COA reductase. After removal of lipopoteins, including LDIL, HMG-COA reductase activities increased some 50- to 100-fold in normal cells (panel a). This high level of enzyme activity was rapidly suppressed upon addition of L.DL back to normal cells (panel b). (b) Cells from FH individuals showed high levels of reductase activity, whether cultured in the presence or absence of LDL. HMG COA Reductase Activity (pmoles/min/mg) LILLI LII
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 2 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Similar questions
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education
SEE MORE TEXTBOOKS