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. In the experiment shown below, fibroblasts obtained from a normal subject (closed symbols) or from a patient homozygous for familial hypercholester- olemia (FH Homozygote) (open symbols) were grown in monolayer cultures. At time zero, the medium was replaced with fresh medium depleted of lipoproteins, and HMG-COA reductase activity was measured in extracts prepared at the indicated times (panel a). Twenty-four hours after addition of the lipoprotein- deficient medium, human LDL was added to the cells at the indicated levels, and HMG-COA reductase activity was measured at the indicated time. AFTER REMOVAL OF LIPOPROTEINS AFTER ADDITION OF LDL 200 FH Homozygote- LDL 100 None - Normal 2 ug/ml 10 20 µg/ml 8. 16 24 32 8. 12 Hours Hours (a) (b) Based on your understanding of HMG-CoA reductase regulation, explain the following results: (a) When cultured in the presence of L.DL, normal cells showed low activity of HMG-COA reductase. After removal of lipopoteins, including LDIL, HMG-COA reductase activities increased some 50- to 100-fold in normal cells (panel a). This high level of enzyme activity was rapidly suppressed upon addition of L.DL back to normal cells (panel b). (b) Cells from FH individuals showed high levels of reductase activity, whether cultured in the presence or absence of LDL. HMG COA Reductase Activity (pmoles/min/mg) LILLI LII

. In the experiment shown below, fibroblasts obtained from a normal subject (closed symbols) or from a patient homozygous for familial hypercholester- olemia (FH Homozygote) (open symbols) were grown in monolayer cultures. At time zero, the medium was replaced with fresh medium depleted of lipoproteins, and HMG-COA reductase activity was measured in extracts prepared at the indicated times (panel a). Twenty-four hours after addition of the lipoprotein- deficient medium, human LDL was added to the cells at the indicated levels, and HMG-COA reductase activity was measured at the indicated time. AFTER REMOVAL OF LIPOPROTEINS AFTER ADDITION OF LDL 200 FH Homozygote- LDL 100 None - Normal 2 ug/ml 10 20 µg/ml 8. 16 24 32 8. 12 Hours Hours (a) (b) Based on your understanding of HMG-CoA reductase regulation, explain the following results: (a) When cultured in the presence of L.DL, normal cells showed low activity of HMG-COA reductase. After removal of lipopoteins, including LDIL, HMG-COA reductase activities increased some 50- to 100-fold in normal cells (panel a). This high level of enzyme activity was rapidly suppressed upon addition of L.DL back to normal cells (panel b). (b) Cells from FH individuals showed high levels of reductase activity, whether cultured in the presence or absence of LDL. HMG COA Reductase Activity (pmoles/min/mg) LILLI LII

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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. In the experiment shown below, fibroblasts obtained from a normal subject (closed symbols) or from a patient homozygous for familial hypercholester- olemia (FH Homozygote) (open symbols) were grown in monolayer cultures. At time zero, the medium was replaced with fresh medium depleted of lipoproteins, and HMG-COA reductase activity was measured in extracts prepared at the indicated times (panel a). Twenty-four hours after addition of the lipoprotein- deficient medium, human LDL was added to the cells at the indicated levels, and HMG-COA reductase activity was measured at the indicated time.
Transcribed Image Text:. In the experiment shown below, fibroblasts obtained from a normal subject (closed symbols) or from a patient homozygous for familial hypercholester- olemia (FH Homozygote) (open symbols) were grown in monolayer cultures. At time zero, the medium was replaced with fresh medium depleted of lipoproteins, and HMG-COA reductase activity was measured in extracts prepared at the indicated times (panel a). Twenty-four hours after addition of the lipoprotein- deficient medium, human LDL was added to the cells at the indicated levels, and HMG-COA reductase activity was measured at the indicated time.
AFTER REMOVAL OF LIPOPROTEINS AFTER ADDITION OF LDL 200 FH Homozygote- LDL 100 None - Normal 2 ug/ml 10 20 µg/ml 8. 16 24 32 8. 12 Hours Hours (a) (b) Based on your understanding of HMG-CoA reductase regulation, explain the following results: (a) When cultured in the presence of L.DL, normal cells showed low activity of HMG-COA reductase. After removal of lipopoteins, including LDIL, HMG-COA reductase activities increased some 50- to 100-fold in normal cells (panel a). This high level of enzyme activity was rapidly suppressed upon addition of L.DL back to normal cells (panel b). (b) Cells from FH individuals showed high levels of reductase activity, whether cultured in the presence or absence of LDL. HMG COA Reductase Activity (pmoles/min/mg) LILLI LII
Transcribed Image Text:AFTER REMOVAL OF LIPOPROTEINS AFTER ADDITION OF LDL 200 FH Homozygote- LDL 100 None - Normal 2 ug/ml 10 20 µg/ml 8. 16 24 32 8. 12 Hours Hours (a) (b) Based on your understanding of HMG-CoA reductase regulation, explain the following results: (a) When cultured in the presence of L.DL, normal cells showed low activity of HMG-COA reductase. After removal of lipopoteins, including LDIL, HMG-COA reductase activities increased some 50- to 100-fold in normal cells (panel a). This high level of enzyme activity was rapidly suppressed upon addition of L.DL back to normal cells (panel b). (b) Cells from FH individuals showed high levels of reductase activity, whether cultured in the presence or absence of LDL. HMG COA Reductase Activity (pmoles/min/mg) LILLI LII
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