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In gel filtration, also known as chromatography, molecules are separated based on their radius which is a function of not only their size but also their Large molecules are excluded from the of

In gel filtration, also known as chromatography, molecules are separated based on their radius which is a function of not only their size but also their Large molecules are excluded from the of

Introduction to Chemical Engineering Thermodynamics
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
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In gel filtration, also known as chromatography, molecules are separated based on their radius which is a function of not only their size but also their . Large molecules are excluded from the of the chromatographic support and therefore elute earlier than molecules. In slide 3.3 of Module 6 Lesson 3, gel filtration and SDS-PAGE experiments were performed under two different conditions: in the presence and the absence of BME and/or DTT. BME stands for B- ethanol while DTT stands for Both are agents that allow the breakage of bonds for the intent to better characterize the high molecular weight (HMW) species (aggregates) in a protein sample. Based on the chromatogram below and the provided areas under the curve (AUC), calculate the amount (percentage) of HMW species found in this sample. Express the value with 2 decimal places. Amount of HMW species = 0.045 0.040 AUC = 97,647 0 035 0.030 0 025 AUC = 3,325 0.020 0 015 0010 Monomer 0 005 HMW
Transcribed Image Text:In gel filtration, also known as chromatography, molecules are separated based on their radius which is a function of not only their size but also their . Large molecules are excluded from the of the chromatographic support and therefore elute earlier than molecules. In slide 3.3 of Module 6 Lesson 3, gel filtration and SDS-PAGE experiments were performed under two different conditions: in the presence and the absence of BME and/or DTT. BME stands for B- ethanol while DTT stands for Both are agents that allow the breakage of bonds for the intent to better characterize the high molecular weight (HMW) species (aggregates) in a protein sample. Based on the chromatogram below and the provided areas under the curve (AUC), calculate the amount (percentage) of HMW species found in this sample. Express the value with 2 decimal places. Amount of HMW species = 0.045 0.040 AUC = 97,647 0 035 0.030 0 025 AUC = 3,325 0.020 0 015 0010 Monomer 0 005 HMW
Based on the chromatogram below and the provided areas under the curve (AUC), calculate the amount (percentage) of HMW species found in this sample. Express the value with 2 decimal places. Amount of HMW species = 0.045 0.040 AUC = 97,647 0.035 O 030 0.025 AUC = 3,325 %3D 0.020 0015 0.010 Monomer 0.005 HMW 0.000 1.00 2.00 3.00 4.00 5 00 6.00 7.00 8.00 9.00 10.00 Minutes Regulatory agencies mandate that HMW species be controlled to less than 5%. Based on the calculation above, does this purified material meet the regulatory requirement? (answer "yes" or "no") The molecular weight of proteins can be determined with SEC by running a series of with known molecular weights to build a curve. SEC a great tool to screen for solutions to find the optimum condition that would promote crystallization of membrane proteins.
Transcribed Image Text:Based on the chromatogram below and the provided areas under the curve (AUC), calculate the amount (percentage) of HMW species found in this sample. Express the value with 2 decimal places. Amount of HMW species = 0.045 0.040 AUC = 97,647 0.035 O 030 0.025 AUC = 3,325 %3D 0.020 0015 0.010 Monomer 0.005 HMW 0.000 1.00 2.00 3.00 4.00 5 00 6.00 7.00 8.00 9.00 10.00 Minutes Regulatory agencies mandate that HMW species be controlled to less than 5%. Based on the calculation above, does this purified material meet the regulatory requirement? (answer "yes" or "no") The molecular weight of proteins can be determined with SEC by running a series of with known molecular weights to build a curve. SEC a great tool to screen for solutions to find the optimum condition that would promote crystallization of membrane proteins.
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