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In gel filtration, also known as chromatography, molecules are separated based on their radius which is a function of not only their size

In gel filtration, also known as chromatography, molecules are separated based on their radius which is a function of not only their size

Introduction to Chemical Engineering Thermodynamics
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
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In gel filtration, also known as chromatography, molecules are separated based on their radius which is a function of not only their size but also their Large molecules are excluded from the of the chromatographic support and therefore elute earlier than molecules. In slide 3.3 of Module 6 Lesson 3, gel filtration and SDS-PAGE experiments were performed under two different conditions: in the presence and the absence of BME and/or DTT. BME stands for 3- ethanol while DTT stands for Both are agents that allow the breakage of bonds for the intent to better characterize the high molecular weight (HMW) species (aggregates) in a protein sample. Based on the chromatogram below and the provided areas under the curve (AUC), calculate the amount (percentage) of HMW species found in this sample. Express the value with 2 decimal places. Amount of HMW species = 0.046 0.040 AUC = 97,647 0.035 0 030 0 026 AUC - 3 315
Transcribed Image Text:In gel filtration, also known as chromatography, molecules are separated based on their radius which is a function of not only their size but also their Large molecules are excluded from the of the chromatographic support and therefore elute earlier than molecules. In slide 3.3 of Module 6 Lesson 3, gel filtration and SDS-PAGE experiments were performed under two different conditions: in the presence and the absence of BME and/or DTT. BME stands for 3- ethanol while DTT stands for Both are agents that allow the breakage of bonds for the intent to better characterize the high molecular weight (HMW) species (aggregates) in a protein sample. Based on the chromatogram below and the provided areas under the curve (AUC), calculate the amount (percentage) of HMW species found in this sample. Express the value with 2 decimal places. Amount of HMW species = 0.046 0.040 AUC = 97,647 0.035 0 030 0 026 AUC - 3 315
amount (percentage) of HMW species found in this sample. Express the value with 2 decimal places. Amount of HMW species D 0.045 0.040 AUC = 97,647 %3D 0.035 0.030 0.025 AUC = 3,325 0.020 0.015 0.010 Monomer 0.005 HMW 0.000 1.00 2.00 3.00 4.00 5,00 6 00 7 00 8.00 9.00 10.00 Minutes Regulatory agencies mandate that HMW species be controlled to less than 5%. Based on the calculation above, does this purified material meet the regulatory requirement? (answer "yes" or "no") The molecular weight of proteins can be determined with SEC by running a series of with known molecular weights to build a curve. SEC a great tool to screen for solutions to find the optimum condition that would promote crystallization of membrane proteins.
Transcribed Image Text:amount (percentage) of HMW species found in this sample. Express the value with 2 decimal places. Amount of HMW species D 0.045 0.040 AUC = 97,647 %3D 0.035 0.030 0.025 AUC = 3,325 0.020 0.015 0.010 Monomer 0.005 HMW 0.000 1.00 2.00 3.00 4.00 5,00 6 00 7 00 8.00 9.00 10.00 Minutes Regulatory agencies mandate that HMW species be controlled to less than 5%. Based on the calculation above, does this purified material meet the regulatory requirement? (answer "yes" or "no") The molecular weight of proteins can be determined with SEC by running a series of with known molecular weights to build a curve. SEC a great tool to screen for solutions to find the optimum condition that would promote crystallization of membrane proteins.
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