In doing a phytochemical determination of natural products, one of the tests involved the determination of indoles. Would the presence of plant secondary metabolites interfere with the tests for proteins giving false-positive results? Why or why not?
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![In doing a phytochemical determination of natural products, one of the tests
involved the determination of indoles. Would the presence of plant secondary
metabolites interfere with the tests for proteins giving false-positive results? Why
or why not?](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F2a01ab9f-23d3-4898-99f4-16f3f504374b%2Fe7fab97f-08cf-4ec7-97be-65d835a44039%2Fwvd1py_processed.png&w=3840&q=75)
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- In phytochemical determination of natural products, one of the tests involve determination of indoles. Would the presence of plant secondary metabolites interfere with the tests for proteins giving false-positive results?In isolating metabolites and testing for phytochemicals, why is it recommended to dry the leaves in open air and not in direct sunlight? Are there other ways to do dry the leaves?In the isolation of metabolites and tests for phytochemicals, why should people dry the leaves in open air and not in direct sunlight? Could there be other ways to do dry the leaves?
- A study was conducted on the impact of phytohormones on medicinal stem explants.The stem explants were cultured onto MS media containingdifferent types of phytohormones as listed in Table below. Predict the resultsobtained after 4 weeks with justifications and conclude the results youpredictedTwo groups of tomatoes were grown under laboratoryconditions, one with humus added to the soil and one acontrol without humus. The leaves of the plants grownwithout humus were yellowish (less green) compared withthose of the plants grown in humus-enriched soil. The bestexplanation is that(A) the healthy plants used the food in the decomposing leavesof the humus for energy to make chlorophyll.(B) the humus made the soil more loosely packed, so waterpenetrated more easily to the roots.(C) the humus contained minerals such as magnesium andiron needed for the synthesis of chlorophyll.(D) the heat released by the decomposing leaves of the humuscaused more rapid growth and chlorophyll synthesis.Switchgrass is used for ethanol production. The composition of the switchgrass is 37% cellulose, 24% xylan, 3% galactan, 4% arabinan, 20% lignin, 7% extractives, and 5% ash. A dilute acid pretreatment method is applied to the switchgrass before enzymatic hydrolysis and fermentation. The pretreatment hydrolyzes 10% hexosan and 90% pentosan into monomeric sugars. Approximately 30% of the hydrolyzed pentoses further react & are decomposed to furfural. Assume that there is no decomposition of the hydrolyzed hexoses. Further Assume that lignin, extractives, and ash do not change during the pretreatment. • How much of each lignocellulosic sugar (glucose, xylose, galactose, and arabinose) is produced when pretreating 1,000 kg (dry matter) switchgrass? How much furfural is formed? • Is water consumed or produced in these pretreatment hydrolysis and dehydration reactions? How much in each?
- You want to prepare 500 mL of the macronutrient and micronutrient stock solutions which have 100X and 1000X higher concentration, respectively, than the required concentration in the culture mediuma)Approximately 4 % (w/v) sucrose and 0.8 % (w/v) agar are added into the medium.Determine the amount of sucrose and agar (in gram) required in order to prepare 200 mL MS medium. Show your workExplain why litmus milk is considered a good differential medium.What initiatives could be taken if plant tissue cultures are suffering severely due to alkaloid production and accumulation in the medium.
- What kind of method of preparation was done? method of preparation (e.g. decoction, infusion, maceration, percolation, etc.)Which would be the control plate for the NaCl experiment and the Sucrose experiment and why?In words, describe the differences you see for each organism grown at 30 C or 37 C (i.e., amount of growth, color, etc)