What kind of method of preparation was done? method of preparation (e.g. decoction, infusion, maceration, percolation, etc.)

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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What kind of method of preparation was done?

method of preparation (e.g. decoction, infusion, maceration, percolation, etc.) 

 

 

Preparation of plant extract
The extracts of leaf and stem of M. quadrifolia were prepared. For this, 20g of leaf and stem powder of M. quadrifolia was soaked in 200 ml of distilled water and
methanol separately and kept in shaker for 48h. After 48h, it was filtered through Whatman no. 1 filter paper and then the volume of supernatant was concentrated
at 40 °C using hot air oven. The concentrated aqueous and methanolic extracts of leaf and stem of M. quadrifolia were used for qualitative phytochemical
analysis. The powdered plant materials were used in the estimation of alkaloids, tannins and saponins.
Transcribed Image Text:Preparation of plant extract The extracts of leaf and stem of M. quadrifolia were prepared. For this, 20g of leaf and stem powder of M. quadrifolia was soaked in 200 ml of distilled water and methanol separately and kept in shaker for 48h. After 48h, it was filtered through Whatman no. 1 filter paper and then the volume of supernatant was concentrated at 40 °C using hot air oven. The concentrated aqueous and methanolic extracts of leaf and stem of M. quadrifolia were used for qualitative phytochemical analysis. The powdered plant materials were used in the estimation of alkaloids, tannins and saponins.
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