In carrying out an analysis of a mixture, what do you expect to see when the TLC plate has been allowed to remain in the developing chamber for too long so that the solvent front has reached the top of the plate?

Q: Which of the following results from an in-line HPLC mobile phase filter becoming plugged with…

A: Correct option is B.

Q: c) diethyl ether. After separating the layers, 240 mg of cyclohexanol are recovered from the diethyl…

A:

Q: How the 2D Thin layer Chromatography separation processes are worked? Please shortly wtite at your…

A: A 2D - TLC plate is a sheet of glass, metal, or plastic which is coated with a thin layer of a solid…

Q: In reversed phase HPLC, how will the retention time of a component of a mixture vary with the…

A: Retention time is a measure of the time that is taken by a solute to pass through a column.In…

Q: How the 2D Thin layer Chromatography separation processes are worked? Please shortly answer at your…

A: Hh

Q: Micellar electrokinetic capillary chromatography was conducted at pH 10 with anionic micelles and…

A: (i) In micellar electrokinetic capillary chromatography, surfactants are added to the buffer…

Q: what is the difference between liquid phase extraction and solid phase extraction

A: A question based on analytical process that is to be accomplished.

Q: What is gradient elution? a. Variation of the column temperature during elution b. Variation of the…

A: Gradient elution chromatography: The separation technique in which components are distributed…

Q: Predict the order of elution for the silica gel adsorption TLC separation of the mixture containing…

A: Silica gel is a polar solvent whereas the solvent methylene chloride is non polar. The polar silica…

Q: In color chromatography of plant pigments, what complications would a dried-out column (solvent…

A: Column chromatography separates molecule mixture based on their differential adsorption on the…

Q: Differentiate between isocratic elution and gradient elution. Why is gradient elution sometimes a…

A:

Q: What controls the mobile phase flow rate in ascending TLC or paper chromatography? Do you think it…

A: The answer is given below

Q: When the use of 2D Thin layer Chromatography is better than normal TLC? Please shortly answer at…

A:

Q: A student was given an unknown compound dissolved in acetone. The student decided to identify the…

A: The unknown compound formed couldn't emit the photons exposed on it at the UV-Region. Therefore, the…

Q: An orange compound, dissolved in dichloromethane, is added to a chromatogra- phy column. The elution…

A: Given:An orange compound is dissolved in dichloromethane.The compound is added to a chromatography…

Q: Of Aspirin, ethanol, acetaminophen, ethyl acetate, hexane, and water which could go undetected…

A: HPLC or high perfomance liquid chromatography is a usefull technique to separate and analyse…

Q: Which of the following is not a method for measuring reaction rates? A) total pressure B) thin layer…

A: A multiple choice question based on measuring reaction rate, which is to be accomplished.

Q: A sample that contained two components was separated on a column. The column had a length of 15.0…

A: Given: length of column = 15 cm initial resolution = 1.2 required resolution = 2.0 we have to find…

Q: Why does the efficiency (decreased plate height) of liquid chromatography increase as the stationary…

A: to improve resolution in HPLC  by Increasing column length. by Decreasing particle size. by…

Q: Analytical Chemistry Describe the formation of plasma and its functions in ICP-MS technique

A: A plasma is essentially an ionised gas, consisting of positively-charged ions and free (unbound)…

Q: A mixture of A and B is injected into a liquid chromatographic column with length of 24.8 cm. The…

A: Given :  Separation resolution between A and B can be calculated using formula: Rs = 2RtB-RtAw1 +…

Q: Explain what will happen when you use 100 microgram of an analyte in a chromatography column which…

A:

Q: Each of the following could lead to poor liquid chromatographic separation. Explain how or why each…

A: Liquid chromatography is a widely used separation technique in chemistry and related fields. It…

Q: In TLC, why does a 3:1:1 ratio of the developing solvent of n-butanol/acetic acid/water move up the…

A: The strength of interactions between adsorbent and adsorbate depends upon the type of interactions…

Q: What are the main differences in column size, operating pressure, sample volume and run time between…

A: Chromatography is a technique  which is used for separating the components, or solute of a mixture…

Q: How does the choice of stationary phase in gas chromatography affect the separation of analytes and…

A: The choice of stationary phase in gas chromatography significantly influences the separation of…

Q: Which of the following factors DOES NOT affect retention time of a sample undergoing normal phase…

A:

Q: In your own words answer the following With reference to the Van Deemter equation, explain…

A: The objective of this question is to explain the Van Deemter equation, explain thoroughly whether…

Q: A mixture of A and B is injected into a liquid chromatographic column with length of 45 cm. The…

A: given- length of the plate= L = 45 cm plate height = H = ?

Q: For each of the following statements, answer TRUE or FALSE and explain your answer briefly.   It…

A: True Potassium Dichromate solutions (235 - 430 nm)The most widely recognised reference material for…

Q: 1) A mixture of benzoic acid, para-xylene and benzaldehyde is spotted on a silica gel TLC plate and…

A:

Q: Describe how retention time of butanol on a poly (ethylene glycol) column will change with…

A: Gas chromatography (GC) is a widely used analytical technique for separating and analyzing volatile…

Q: Indicate the order of elution in which the following compounds would be eluted from an HPLC column…

A: Concept: In reversed phase packing HPLC more polar compound will elute first. So we have to know the…

Q: What is the concentration meant by inner filter effects?

A: The concentration for inner filter effects .

Q: If retention times found on a chromatogram are 0.60 min for CH4 (=tm), 7.0 min for pentane, 8.0 min…

A: We use the formula below to find Kovat's retention index(I): Kovat's retention index, I = 100*n +…

Q: 7. Explain why the level of cluant in the TLC chamber should never reach the line where the mixture…

A: Thin layer chromatography is a separation technique where the stationary phase is silica which is…

Q: Using thin-layer chromatography showed that the black dye used in a brand of writing ink contained…

A: The Rf value is defined as the ratio of the distance moved by the solute and the distance moved by…

Q: List 3 criteria that is important to consider when choosing a recrystallization solvent   In a TLC…

A: Recrystallization is an experimental technique of separating pure compound from the solution…

Q: Explain the importance of controlling mobile phase pH in reversed phase HPLC and how this is…

A: Please find your solution below : Most of the compounds analysed by HPLC contain one or more acidic…

Q: How does increasing the polarity of the developing solvent affect the Rf of a compound on a silica…

A: TLC stands for Thin Layer Chromatography. This process is basically used for the differentiation of…