In a fluorescence-lifetime experiment, a very short pulse of light is used to excite a sample. Then the intensity of the fluorescence is recorded as a function of time. Suppose we study a dansyl fluorophore. The intensity of the fluorescent light versus time is as follows: Fluorescence intensity Time (ns) 1.1 x 104 4.9 x 103 5 2.3 x 103 10 1.3 x 103 15 5.6 x 102 20 Part A What is the observed fluorescence lifetime? Express your answer to two significant figures and include the appropriate units. T= 6.9 ns Submit Previous Answers Correct

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In a fluorescence-lifetime experiment, a very short pulse
of light is used to excite a sample. Then the intensity of
the fluorescence is recorded as a function of time.
Suppose we study a dansyl fluorophore. The intensity of
the fluorescent light versus time is as follows:
Fluorescence intensity Time (ns)
1.1 x 104
4.9 x 103
5
2.3 x 103
10
1.3 x 103
15
5.6 x 102
20
Part A
What is the observed fluorescence lifetime?
Express your answer to two significant figures and
include the appropriate units.
T = 6.9 ns
Submit
Previous Answers
Correct
Part B
The fluorescence quantum yield under the same
conditions was determined to be 0.7. What is the
intrinsic rate constant for radiative fluorescence decay?
Express your answer to two significant figures and
include the appropriate units.
НА
?
Value
TO
Units
Submit
Previous Answers Request Answer
X Incorrect; Try Again; 4 attempts
remaining
Part C
It has been determined separately that when the dansyl
chromophore is 20 A away from the 11-cis-retinal
chromophore in rhodopsin, the efficiency of
fluorescence energy transfer is 50%. In a second
experiment, rhodopsin is covalently labeled with a
dansyl chromophore and the observed fluorescence
lifetime for the dansyl-rhodopsin complex is 6 ns. What
is the distance between the dansyl label and the retinal
chromophore in rhodopsin?
Express your answer to two significant figures and
include the appropriate units.
HA
?
Valuo
Transcribed Image Text:In a fluorescence-lifetime experiment, a very short pulse of light is used to excite a sample. Then the intensity of the fluorescence is recorded as a function of time. Suppose we study a dansyl fluorophore. The intensity of the fluorescent light versus time is as follows: Fluorescence intensity Time (ns) 1.1 x 104 4.9 x 103 5 2.3 x 103 10 1.3 x 103 15 5.6 x 102 20 Part A What is the observed fluorescence lifetime? Express your answer to two significant figures and include the appropriate units. T = 6.9 ns Submit Previous Answers Correct Part B The fluorescence quantum yield under the same conditions was determined to be 0.7. What is the intrinsic rate constant for radiative fluorescence decay? Express your answer to two significant figures and include the appropriate units. НА ? Value TO Units Submit Previous Answers Request Answer X Incorrect; Try Again; 4 attempts remaining Part C It has been determined separately that when the dansyl chromophore is 20 A away from the 11-cis-retinal chromophore in rhodopsin, the efficiency of fluorescence energy transfer is 50%. In a second experiment, rhodopsin is covalently labeled with a dansyl chromophore and the observed fluorescence lifetime for the dansyl-rhodopsin complex is 6 ns. What is the distance between the dansyl label and the retinal chromophore in rhodopsin? Express your answer to two significant figures and include the appropriate units. HA ? Valuo
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