Ily transfer your gel to wrap. Take a photograph of your gel for your records and lab report. Completely cover your gel with a layer of plastic wrap. 3. Measure the distance travelled by the dye front. DYE FRONT = 54 mm 4. Measure in mm (not cm) the distance travelled (or "migration distance") for each protein in your ladder. Measure from the bottom of the well to each band. 5. Use the Excel Tutorial Video on graphing and solving for the kD sizes of the unknowns. Protein Ladder (for trendline graph): Migration Rf= Distance Band Migration Dye Front Migration 0-074 (mm) 4 17 22. 28. 31 Measurements for Unknown Migration Distance (mm) ما 0-166 0.203 0.314 0-407 esig 8.50.106 017591 4 14 Molecular Weight (in kD) Rf 6074 260 250 CONCESS 10 |||||||||| kDa -260- --140- -100- -70 -50 -40 -35 -25 -15 -10 Log of Molecular Weight 2.415 217 2 187 tet 1.57 1.4 1.3 Estimated Molecular Weight

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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Can you please help me to make a professional table with excel I don’t know how to use exell thanks you
wrap. Take a photograph of your gel for your records and lab report. Completely cover your gel
light box. Carefully transfer your gel to the plastic
with a layer of plastic wrap.
3. Measure the distance travelled by the dye front. DYE FRONT =
mm
4. Measure in mm (not cm) the distance travelled (or "migration distance") for each protein in
54
your ladder. Measure from the bottom of the well to each band.
5. Use the Excel Tutorial Video on graphing and solving for the kD sizes of the unknowns.
Protein Ladder (for trendline graph):
Migration
Rf=
Distance
Band Migration
Dye Front Migration
0-074
0-111
0-166
(mm)
4
17
22,
28.
31
0.203
0.314
0-407
esig
1 14
574
0.759
Measurements for Unknown
Migration Distance (mm)
Molecular Weight
(in KD)
260 250.
Rf
•074
站
200
1|||||||||
kDa
-260-
-140-
-100.
-70
-50
-40
-35
-25
-15
-10-
Log of
Molecular Weight
2.415
217
2
187
1.57
1.4
1.3
Estimated Molecular Weight
Transcribed Image Text:wrap. Take a photograph of your gel for your records and lab report. Completely cover your gel light box. Carefully transfer your gel to the plastic with a layer of plastic wrap. 3. Measure the distance travelled by the dye front. DYE FRONT = mm 4. Measure in mm (not cm) the distance travelled (or "migration distance") for each protein in 54 your ladder. Measure from the bottom of the well to each band. 5. Use the Excel Tutorial Video on graphing and solving for the kD sizes of the unknowns. Protein Ladder (for trendline graph): Migration Rf= Distance Band Migration Dye Front Migration 0-074 0-111 0-166 (mm) 4 17 22, 28. 31 0.203 0.314 0-407 esig 1 14 574 0.759 Measurements for Unknown Migration Distance (mm) Molecular Weight (in KD) 260 250. Rf •074 站 200 1||||||||| kDa -260- -140- -100. -70 -50 -40 -35 -25 -15 -10- Log of Molecular Weight 2.415 217 2 187 1.57 1.4 1.3 Estimated Molecular Weight
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