Ily transfer your gel to wrap. Take a photograph of your gel for your records and lab report. Completely cover your gel with a layer of plastic wrap. 3. Measure the distance travelled by the dye front. DYE FRONT = 54 mm 4. Measure in mm (not cm) the distance travelled (or "migration distance") for each protein in your ladder. Measure from the bottom of the well to each band. 5. Use the Excel Tutorial Video on graphing and solving for the kD sizes of the unknowns. Protein Ladder (for trendline graph): Migration Rf= Distance Band Migration Dye Front Migration 0-074 (mm) 4 17 22. 28. 31 Measurements for Unknown Migration Distance (mm) ما 0-166 0.203 0.314 0-407 esig 8.50.106 017591 4 14 Molecular Weight (in kD) Rf 6074 260 250 CONCESS 10 |||||||||| kDa -260- --140- -100- -70 -50 -40 -35 -25 -15 -10 Log of Molecular Weight 2.415 217 2 187 tet 1.57 1.4 1.3 Estimated Molecular Weight
Ily transfer your gel to wrap. Take a photograph of your gel for your records and lab report. Completely cover your gel with a layer of plastic wrap. 3. Measure the distance travelled by the dye front. DYE FRONT = 54 mm 4. Measure in mm (not cm) the distance travelled (or "migration distance") for each protein in your ladder. Measure from the bottom of the well to each band. 5. Use the Excel Tutorial Video on graphing and solving for the kD sizes of the unknowns. Protein Ladder (for trendline graph): Migration Rf= Distance Band Migration Dye Front Migration 0-074 (mm) 4 17 22. 28. 31 Measurements for Unknown Migration Distance (mm) ما 0-166 0.203 0.314 0-407 esig 8.50.106 017591 4 14 Molecular Weight (in kD) Rf 6074 260 250 CONCESS 10 |||||||||| kDa -260- --140- -100- -70 -50 -40 -35 -25 -15 -10 Log of Molecular Weight 2.415 217 2 187 tet 1.57 1.4 1.3 Estimated Molecular Weight
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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