II. Objectives: In this activity, we should be able to: A. extract DNA from a fruit; B. view the extracted DNA in a microscope; and C. document and accomplish the experiment paper. III. Materials: heavy duty Ziploc bag (quart size) 1 ripe strawberry or 1 slice ripe banana 2 teaspoons of detergent 1 teaspoon of salt Gauze pad (for filtering the solution) 120 mL of water glass stirring rod inoculating loop apparatus to stir DNA 10 mL ice-cold rubbing alcohol (91% works best) 1 50 mL beaker Graduated cylinder (10ml) - microscope cover slip glass slide funnel - 2 test tube - I test to be tack i spatua IV. Procedure: 1. Place one slice of fruit in a Ziploc bag. 2. Smash/grind up the fruit using your fist and fingers for 2 minutes. Be careful not to break the bag. 3. In a beaker, make the DNA extraction fluid. Mix 2 teaspoons of detergent, 1 teaspoon of salt, and ½ cup of water. 4. Add 2 teaspoons of the DNA extraction fluid into the bag. 5. Kneed/mush the fruit in the bag again for 1 minute. Avoid making too many soap bubbles). 6. Assemble your filtration apparatus. Place your test tube into a test tube rack to keep it stable. Place the gauze pad over the funnel and pour the fruit mixture. 7. Gently squeeze the cloth to drain the extract. Discard the left-over chunks of fruit on the gauge pad. 8. Carefully tilt the test tube and slowly pour cold rubbing alcohol equal to the amount of extract, DO NOT quickly dump the alcohol straight into the fruit liquid. 9. Wait about 2-3 minutes and you should start to see white clumps and strings forming, that is the DNA. 10. Using the inoculating loop, gently pick up a strand of DNA. +1. Place the strand of DNA on a glass slide, and cover it using the cover slip. 13 Focus the
II. Objectives: In this activity, we should be able to: A. extract DNA from a fruit; B. view the extracted DNA in a microscope; and C. document and accomplish the experiment paper. III. Materials: heavy duty Ziploc bag (quart size) 1 ripe strawberry or 1 slice ripe banana 2 teaspoons of detergent 1 teaspoon of salt Gauze pad (for filtering the solution) 120 mL of water glass stirring rod inoculating loop apparatus to stir DNA 10 mL ice-cold rubbing alcohol (91% works best) 1 50 mL beaker Graduated cylinder (10ml) - microscope cover slip glass slide funnel - 2 test tube - I test to be tack i spatua IV. Procedure: 1. Place one slice of fruit in a Ziploc bag. 2. Smash/grind up the fruit using your fist and fingers for 2 minutes. Be careful not to break the bag. 3. In a beaker, make the DNA extraction fluid. Mix 2 teaspoons of detergent, 1 teaspoon of salt, and ½ cup of water. 4. Add 2 teaspoons of the DNA extraction fluid into the bag. 5. Kneed/mush the fruit in the bag again for 1 minute. Avoid making too many soap bubbles). 6. Assemble your filtration apparatus. Place your test tube into a test tube rack to keep it stable. Place the gauze pad over the funnel and pour the fruit mixture. 7. Gently squeeze the cloth to drain the extract. Discard the left-over chunks of fruit on the gauge pad. 8. Carefully tilt the test tube and slowly pour cold rubbing alcohol equal to the amount of extract, DO NOT quickly dump the alcohol straight into the fruit liquid. 9. Wait about 2-3 minutes and you should start to see white clumps and strings forming, that is the DNA. 10. Using the inoculating loop, gently pick up a strand of DNA. +1. Place the strand of DNA on a glass slide, and cover it using the cover slip. 13 Focus the
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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What is the purpose of the detergent in the experiment?
Transcribed Image Text:DNA from a truit.
II. Objectives:
In this activity, we should be able to:
extract DNA from a fruit;
A.
B.
view the extracted DNA in a microscope; and
C. document and accomplish the experiment paper.
III. Materials:
heavy duty Ziploc bag (quart size)
1 ripe strawberry or 1 slice ripe banana
2 teaspoons of detergent
1 teaspoon of salt
Gauze pad (for filtering the solution)
120 mL of water
glass stirring rod
inoculating loop apparatus to stir DNA
10 mL ice-cold rubbing alcohol (91% works best)
1 50 mL beaker
Graduated cylinder (10ml)
- microscope
cover slip
> glass slide
funnel
- 2 test tube
- I test to be tack
i spatula
IV. Procedure:
1. Place one slice of fruit in a Ziploc bag.
2. Smash/grind up the fruit using your fist and fingers for 2 minutes. Be careful not to break the
bag.
3. In a beaker, make the DNA extraction fluid. Mix 2 teaspoons of detergent, 1 teaspoon of
salt, and ½ cup of water.
4. Add 2 teaspoons of the DNA extraction fluid into the bag.
5. Kneed/mush the fruit in the bag again for 1 minute. Avoid making too many soap bubbles).
6. Assemble your filtration apparatus. Place your test tube into a test tube rack to keep it
stable. Place the gauze pad over the funnel and pour the fruit mixture.
7. Gently squeeze the cloth to drain the extract. Discard the left-over chunks of fruit on the
gauge pad.
8. Carefully tilt the test tube and slowly pour cold rubbing alcohol equal to the amount of
extract, DO NOT quickly dump the alcohol straight into the fruit liquid.
9. Wait about 2-3 minutes and you should start to see white clumps and strings forming, that is
the DNA.
10. Using the inoculating loop, gently pick up a strand of DNA.
+1. Place the strand of DNA on a glass slide, and cover it using the cover slip.
12. Focus the prepared slide under LPO then shift to HPQ to observe the detailed structure
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