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(ii) IFXI gene is inserted at Scal restriction site of the plasmid, what is the difficulty of the screening process to select the potential recombinants?

(ii) IFXI gene is inserted at Scal restriction site of the plasmid, what is the difficulty of the screening process to select the potential recombinants?

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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answer ii)

PUC18 (Figure 2) is a plasmid cloning vector commonly used with Escherichia coli (E. coli). Eco01091 2874 Plol 46 BotAPI 179 Aatil 2017 Sapl 250 Ndel 183 Ehel 235 Pdaml 2204 Bogl 215 Scal 217 MCS lacz 14/ PUC18 2686 bp Baa 6s0 Sapi e83 AIIL, Pecl 800 Gsul 1784 Ctriol 1779 Eco311 1766. Eaml 105 1004 rep (pMB1) \Cail 1217 \Bsel 1110 Hinc C Acc Eco EcoRI Xapi 456 SG TAA AAC GAC GạC CAG Tạc CAA GCT TGC ATG CCT GCA GGT CGA CTC TAG AGG ATCC ccc GGG TAC CGA GCT CGA ATT CGT aCATT TTG CTa ccG aTC ACG GTT CGA ACG TAC GGA CGT CCA OCT GAG ATC TCC TAG GGG CCC ATG OCT CGA GCT TAA GCA Gu Leu Pro Asp Gly Pre Val Ser Ser Ser Asn The Ecota MuC ncn (a ind Pael Sdalkl Xmil Xhal Bam SmalKpnl Saci Lacz Val val Ala Leu Ala Ser Ala His Arg Cys The AAT CAT GGT CAT AGC TGT TTC CTG 3 TTA GTA CCA GTA TCG ACA AAG GAC S e Met The Met yic eenngp . 17m Figure 2 XI gene was inserted at the Hindlll and EcoRI restriction sites of the plasmid illustrated in Figure 2. Describe the screening process to select the positive recombinants. (i) If XI gene is inserted at Scal restriction site of the plasmid, what is the difficulty of the screening process to select the potential recombinants? (ii) bla (Ap)
Transcribed Image Text:PUC18 (Figure 2) is a plasmid cloning vector commonly used with Escherichia coli (E. coli). Eco01091 2874 Plol 46 BotAPI 179 Aatil 2017 Sapl 250 Ndel 183 Ehel 235 Pdaml 2204 Bogl 215 Scal 217 MCS lacz 14/ PUC18 2686 bp Baa 6s0 Sapi e83 AIIL, Pecl 800 Gsul 1784 Ctriol 1779 Eco311 1766. Eaml 105 1004 rep (pMB1) \Cail 1217 \Bsel 1110 Hinc C Acc Eco EcoRI Xapi 456 SG TAA AAC GAC GạC CAG Tạc CAA GCT TGC ATG CCT GCA GGT CGA CTC TAG AGG ATCC ccc GGG TAC CGA GCT CGA ATT CGT aCATT TTG CTa ccG aTC ACG GTT CGA ACG TAC GGA CGT CCA OCT GAG ATC TCC TAG GGG CCC ATG OCT CGA GCT TAA GCA Gu Leu Pro Asp Gly Pre Val Ser Ser Ser Asn The Ecota MuC ncn (a ind Pael Sdalkl Xmil Xhal Bam SmalKpnl Saci Lacz Val val Ala Leu Ala Ser Ala His Arg Cys The AAT CAT GGT CAT AGC TGT TTC CTG 3 TTA GTA CCA GTA TCG ACA AAG GAC S e Met The Met yic eenngp . 17m Figure 2 XI gene was inserted at the Hindlll and EcoRI restriction sites of the plasmid illustrated in Figure 2. Describe the screening process to select the positive recombinants. (i) If XI gene is inserted at Scal restriction site of the plasmid, what is the difficulty of the screening process to select the potential recombinants? (ii) bla (Ap)
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