If you expose human tissue culture cells (for example,HeLa cells) to 3H-thymidine just as they enter Sphase, then wash this material off the cells and letthem go through a second S phase before looking atthe chromosomes, how would you expect the 3H to bedistributed over a pair of homologous chromosomes?(Ignore the effect recombination could have on thisoutcome.) Would the radioactivity be in (a) one chromatid of one homolog, (b) both chromatids of one homolog, (c) one chromatid each of both homologs,(d) both chromatids of both homologs, or (e) someother pattern? Choose the correct answer and explainyour reasoning. (This problem extends the analysisbegun in Solved Problem III.)

If you expose human tissue culture cells (for example,HeLa cells) to 3H-thymidine just as they enter Sphase, then wash this material off the cells and letthem go through a second S phase before looking atthe chromosomes, how would you expect the 3H to bedistributed over a pair of homologous chromosomes?(Ignore the effect recombination could have on thisoutcome.) Would the radioactivity be in (a) one chromatid of one homolog, (b) both chromatids of one homolog, (c) one chromatid each of both homologs,(d) both chromatids of both homologs, or (e) someother pattern? Choose the correct answer and explainyour reasoning. (This problem extends the analysisbegun in Solved Problem III.)

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter8: The Structure, Replication, And Chromosomal Organization Of Dna

Section: Chapter Questions

Problem 6QP: Recently, scientists discovered that a rare disorder called polkadotism is caused by a bacterial...

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If you expose a culture of human cells (for example,HeLa cells) to 3H-thymidine during S phase, howwould the radioactivity be distributed over a pair ofhomologous chromosomes at metaphase? Would theradioactivity be in (a) one chromatid of one homolog,(b) both chromatids of one homolog, (c) one chromatid each of both homologs, (d) both chromatids ofboth homologs, or (e) some other pattern? Choose thecorrect answer and explain your reasoning.
Roman 16px : 1.0pt : BIUS A A X2 x E tion of chromosomes did not occur berore cytoknESIS, 1/2 the # of chromsomes or 1 of each b. If the situation in part a occurred, would the new cells be viable? Explain. no, it wouldnt have the DNA OP 10. The S phase stands for synthesis, which means to make or build something more complex out of simpler parts. Scientists know that during the S phase DNA is being made in the nucleus of the cell. Why do you think the cell needs to make more DNA at this time in the cell cycle? 11. Refer to Model 1. The chromosomes that are shaped like "X" (made of two sister chromatids) have double the amount of DNA than the chromosomes that are shaped like "I." During what phase of the cell cycle do you think the chromosomes are replicated (copied)? POGIL Activities for High School Biology 2. hp
Acridine orange is a chemical that inhibits the replication ofF- factor DNA but does not affect the replication of chromosomalDNA, even if the chromosomal DNA contains an Hfr. Let’s supposethat you have an E. coli strain that is unable to metabolizelactose and has an F factor that carries a streptomycin-resistantgene. You also have an F− strain of E. coli that is sensitive to streptomycinand has the genes that allow the bacterium to metabolize lactose. This second strain can grow on a lactose-containing medium.How would you generate an Hfr strain that is resistant tostreptomycin and can metabolize lactose? (Hint: F factors occasionally integrate into the chromosome to become Hfr strains, and occasionally Hfr strains excise their DNA from the chromosome to become F+ strains that carry an F′ factor.)
Read the statements below and determine which f the recombination mechanisms that it matches with - there could be more than one match or there could be no match at all. For each box, place a YES or NO in the box. Statement 1. Donor cell produces a pilus in order to exchange DNA with recipient cell. 2. Mediated by a bacteriophage. 3. Always involves a plasmid. 4. Allows recombination of chromosomal DNA fragments from donor to recipient. 5. Requires rolling circle replication. 6. Requires donor to recipient cell direct contact. 7. Allows the uptake of free DNA fragments from the environment. 8. Is always horizontal gene exchange. 9. Allows movement of genes from a plasmid to a chromosome. 10. Requires a donor cell to infect a recipient cell in order for recombination to occur. 11. Involves plasmid integration into the chromosome prior to recombination. 12. Involves incorrect packaging of donor DNA into a phage particle in order for recombination to occur. 13. Involves DNA that was…
When the interrupted mating technique was used withfive different strains of Hfr bacteria, the following orders ofgene entry and recombination were observed. On the basisof these data, draw a map of the bacterial chromosome.Do the data support the concept of circularity?HfrStrain Order1 T C H R O2 H R O M B3 M O R H C4 M B A K T5 C T K A B
The cells from a person’s malignant tumor were subjected to insitu hybridization using a probe that recognizes a unique sequenceon chromosome 14. The probe was detected only once in each ofthe cells. Explain this result, and speculate on its significance withregard to the malignant characteristics of these cells.
If you extract the DNA of the coliphage φ X174, you willfind that its composition is 25 percent A, 33 percent T,24 percent G, and 18 percent C. Does this compositionmake sense in regard to Chargaff’s rules? How wouldyou interpret this result? How might such a phage replicate its DNA
In an electrophoretic gel across which is applied a powerful electrical alternating pulsed field, the DNA of thehaploid fungus Neurospora crassa (n = 7) moves slowlybut eventually forms seven bands, which represent DNAfractions that are of different sizes and hence havemoved at different speeds. These bands are presumed tobe the seven chromosomes. How would you show whichband corresponds to which chromosome?
By conducting conjugation experiments between Hfr and recipientstrains, Wollman and Jacob mapped the order of many bacterialgenes. Throughout the course of their studies, they identified severaldifferent Hfr strains in which the F-factor DNA had been integratedat different places along the bacterial chromosome. A sample oftheir experimental results is shown in the following table: A. E xplain how these results are consistent with the idea that thebacterial chromosome is circular.B. Draw a map of the bacterial chromosome that shows the orderof genes and the locations of the origins of transfer among thesedifferent Hfr strains.
In a bacterial culture in which all cells are unable to synthesizeleucine (leu-), a potent mutagen is added, and the cells areallowed to undergo one round of replication. At that point, samplesare taken, a series of dilutions are made, and the cells areplated on either minimal medium or minimal medium containingleucine. The first culture condition (minimal medium) allowsthe growth of only leu+ cells, while the second culture condition(minimal medium with leucine added) allows growth of all cells.The results of the experiment are as follows: Culture Condition Dilution ColoniesMinimal medium 10-1 18Minimal medium + leucine 10-7 6What is the rate of mutation at the locus associated with leucinebiosynthesis?
. E. coli chromosomes in which every nitrogen atom is labeled (that is, every nitrogen atom is the heavy isotope15N instead of the normal isotope 14N) are allowed to replicate in an environment in which all the nitrogen is 14N.Using a solid line to represent a heavy polynucleotidechain and a dashed line for a light chain, sketch each ofthe following descriptions:a. The heavy parental chromosome and the productsof the first replication after transfer to a 14N medium,assuming that the chromosome is one DNA doublehelix and that replication is semiconservative.b. Repeat part a, but now assume that replication isconservative.c. If the daughter chromosomes from the first divisionin 14N are spun in a cesium chloride density gradientand a single band is obtained, which of the possibilitiesin parts a and b can be ruled out? Reconsider theMeselson and Stahl experiment: What does it prove?
I am trying to understand DNA/chromosome structure and replication. I have a culture dish of human cells (NIH3T3) and expose them to radioactively labeled Thymidine, 3H-thymidine, during S phase of the cell cycle. How would my radioactivity be distributed over a pair of homologous chromosomes at metaphase? If i have to draw How the radioactivity would be distributed over a pair of homologous chromosomes at metaphase, how will my drwing look like