If you are making 500 ml of a stock solution of 10X PBS pH7.4 please tell me how much of each  component you would need to add if the appropriate concentrations of 1x PBS are:  1x PBS Solution Concentrations:  NaCl: 137 mM  KCl: 2.7 mM  Na2HPO4: 10 mM  KH2PO4: 1.8 mM  MW NaCL= 58.44 g/mol  MW KCl = 74.55 g/mol  MW Na2HPO4= 141.96 g/mol  MW KH2PO4= 136.09 g/mol  You should also describe the step by step process of making the solution.

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Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
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If you are making 500 ml of a stock solution of 10X PBS pH7.4 please tell me how much of each  component you would need to add if the appropriate concentrations of 1x PBS are: 

1x PBS Solution Concentrations: 

NaCl: 137 mM 

KCl: 2.7 mM 

Na2HPO4: 10 mM 

KH2PO4: 1.8 mM 

MW NaCL= 58.44 g/mol 

MW KCl = 74.55 g/mol 

MW Na2HPO4= 141.96 g/mol 

MW KH2PO4= 136.09 g/mol 

You should also describe the step by step process of making the solution.



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