If  HCO, is 24 mEq/L, and PaCO2  is 40 mmHg calculate the pH.

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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If  HCO, is 24 mEq/L, and PaCO is 40 mmHg calculate the pH.

Expert Solution
Step 1

The Henderson-Hasselbalch equation describes the relationship of pH as a measure of acidity with the acid dissociation constant (Ka) in biological and chemical systems. The equation is especially useful in estimating the pH of a buffer solution and finding equilibrium pH in acid-base reactions.

Step 2

The given question has carbonic acid buffer system whose equilibrium equation can be given as:

H++HCO3-CO2+H2O

The pH by Henderson-Hasselbalch equation for the above reaction can be given as:

pH=pKa+log[HCO3-][CO2]

pKa for bicarbonate buffer system is 6.1. [HCO3-]=24 mEq/L and pCO2=40 mmHg is given. The pH can be calculated by the above equation as follows:

[CO2]=0.03×pCO2pH=6.1+log2440×0.03pH=6.1+log(20)pH=6.1+1.30=7.4

 

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