If an unknown bacteria was given that was gram-positive coccus, and the catalase test was performed, what can be determined from the result of the test?
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If an unknown bacteria was given that was gram-positive coccus, and the catalase test was performed, what can be determined from the result of the test?
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- a) explain in your own words how to do a nitrate reduction test to identify unknown bacteria b) explain in your own words how to perform a phenol red broth (prb) test to help identify unknown bacteriaYou perform the Methyl-Red (right) and Voges-Proskauer test (left). Interpret the results. Which methods would you use to determine is the bacterial sample is E. coli or P. vulargis? What results would help you to decide?in the clinic, the acid-fast stain is used to diagnose infection with which bacterium?
- If no amplified product was visualized by UV light on the gel, does it mean that there is no water-borne microbial pathogen in the unknown sample?When using Endo agar as the selective and differential media for the confirmed test, how would you know whether the test is positive for the presence of coli?After the original specimen has been plated and a reported gram-negative rod was seen on the initial gram stain, what is your initial assumption of the organism causing this disease?
- What was the concentration of bacteria (CFU/mL) in the original suspensions used for dilutions and plating below: Dilution Amount plated # of colonies counted 10-4 1.0 mL 271 10-5 0.1 mL 36 10-6 1.0 mL 9A culture of Staphylococcus is diluted as follows:(1) 20mL are added to 80mL of water.(2) 10uL from (1) are then added to 9.99mL of water.(3) A 10-2 dilution is made from tube # (2).(4) 100uL from (3) are plated for a pour plate and incubated. There were 34 colonies counted on one quarter of the plate following incubation. a) What was the overall dilution?b) How many cfu/mL were present in the original culture?Describe one error in the staining procedure that would result in a Gram-positive bacterium appearing pink.