how to use valine in a 2 chemical equation involved in this experiment using the scheme below . HPLC or ion-exchangechromatography6N HCIFree aminoacidsAmino acidcomposition(a)Determine types and amountsof amino acids in polypeptide.PolypeptideNO,NO2NO.FDNBNO,NHNO,NONHFree+ aminoacids-R CH6 M HCIIdentify amino-terminalresidue of polypeptide.R-CHC-0CO-2,4-Dinitro-phenylderivativeof polypeptide2,4-Dinitrophenylderivativeof amino-terminalresidueHNR-CHC=0

A method for separating and analyzing amino acids in a mixture is HPLC Ion Exchange Chromatography.…

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Biochemistry

how to use valine in a 2 chemical equation involved in this experiment using the scheme below .

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

how to use valine in a 2 chemical equation involved in this experiment using the scheme below .

HPLC or ion-exchange
chromatography
6N HCI
Free amino
acids
Amino acid
composition
(a)
Determine types and amounts
of amino acids in polypeptide.
Polypeptide
NO,
NO2
NO.
FDNB
NO,
NH
NO,
NO
NH
Free
+ amino
acids
-R CH
6 M HCI
Identify amino-terminal
residue of polypeptide.
R-CH
C-0
CO-
2,4-Dinitro-
phenyl
derivative
of polypeptide
2,4-Dinitrophenyl
derivative
of amino-terminal
residue
HN
R-CH
C=0

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Step 1: Explain Ion Exchange Chromatography in HPLC

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Step 2: Identify the Amino Acids in Polypeptide

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Step 3: Predict the 2,4-Dinitrophenyl (DNP) Derivative

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